Platelet Adhesion to Collagen Type I, Collagen Type IV, von Willebrand Factor, Fibronectin, Laminin and Fibrinogen: Rapid Kinetics under Shear
Platelet Adhesion to Collagen Type I, Collagen Type IV, von Willebrand Factor, Fibronectin, Laminin and Fibrinogen: Rapid Kinetics under Shear
复制标题
血小板对 I 型胶原蛋白、IV 型胶原蛋白、血管性血友病因子、纤连蛋白、层粘连蛋白和纤维蛋白原的粘附：剪切下的快速动力学
作者：
R. Polanowska;C. Simon;A. Gear
血管壁的细胞外基质蛋白通过促进血管损伤部位的血小板粘附，在止血中发挥重要作用。我们将连续流动系统与亲和色谱相结合，研究了模拟动脉流动条件下血小板的粘附，并检测了未受刺激的血小板对I型和IV型胶原、血管性血液病因子（vWf）、纤维连接蛋白、层粘连蛋白和纤维蛋白原的粘附动力学。在没有红细胞的情况下，acd制备的血浆与I型和IV型胶原或vWf的粘附是快速有效的（在<1 s内达到50%），并且与650至3400 s-1的剪切速率无关，动力学遵循反指数衰减曲线。我们介绍了一个简单的数学模型，其中这种类型的动力学产生，它可能更普遍地适用于流动条件下的各种粘附过程。该模型的特点是粘附表面的血小板沉积速率与流动中血小板的数量成正比。纤维连接蛋白的粘附与剪切速率无关，但在显著粘附开始前显示出约1.5 s的滞后期。在低剪切速率（650-1000 s-1）下，层粘连蛋白和纤维蛋白原支持有效的粘附，但在高剪切速率（1700-3400 s-1）下，阻滞期为~1.5 s。对照蛋白（白蛋白和明胶）支持最小的粘附。与其他底物蛋白不同，聚赖氨酸的非特异性粘附动力学是线性的。总之，在流动条件下，人血小板特异性地、快速地（在几秒内）和有效地粘附在几种蛋白质上，粘附动力学取决于作为底物的蛋白质以及剪切速率。
Summary Extracellular matrix proteins in the blood vessel wall fulfill an essential role in haemostasis by promoting platelet adhesion at the site of vessel injury. We have combined a continuous-flow system with affinity chromatography to study platelet adhesion under conditions mimicking arterial flow and have examined the adhesion kinetics of unstimulated platelets to collagens type I and IV, von Willebrand factor (vWf), fibronectin, laminin and to fibrinogen. In the absence of red cells, in ACD-prepared plasma adhesion to collagens type I and IV or vWf was rapid, efficient (>50% in <1 s ) and independent of shear rates from 650 to 3400 s-1with kinetics following an inverse exponential decay curve. We introduced a simple mathematical model in which this type of kinetics arises, and which may be more generally applicable to various adhesion processes under flow conditions. The model is characterized by the rate of platelet deposition on the adhesive surface being proportional to the number of platelets in the flow. Adhesion to fibronectin was independent of shear rate, but revealed a lag phase of ~1.5 s before significant adhesion began. Laminin and fibrinogen supported efficient adhesion at low shear rates (650-1000 s-1), but a lag phase of ~1.5 s was seen at high shear rates (1700-3400 s-1). Control proteins (albumin and gelatin) supported minimal adhesion. Nonspecific adhesion to poly-l-lysine differed from that to other substrate proteins in that the kinetics were linear. In conclusion, human platelets adhered specifically, rapidly (within seconds) and efficiently to several proteins under flow conditions and the kinetics of adhesion depended on the protein serving as substrate as well as on shear rate.
测定血小板与胶原蛋白的粘附以及相关的磷脂酸形成和钙动员。
影响因子：
2.9
作者：
Smith,JB;Dangelmaier,C
通讯作者：
Dangelmaier,C
影响因子：
20.3
作者：
Tabish;Zaidi;Larry;Mclntire;David;Farrell;Perumal Thiagarajan
通讯作者：
Tabish;Zaidi;Larry;Mclntire;David;Farrell;Perumal Thiagarajan
研究血小板与胶原蛋白粘附的定量方法。
DOI：
--
发表时间：
1984
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Aihara,M;Cooper,HA;Wagner,RH
通讯作者：
Wagner,RH