A review of tandem mass spectrometry characterization of adenosine diphosphate-ribosylated peptides
A review of tandem mass spectrometry characterization of adenosine diphosphate-ribosylated peptides
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DOI：
10.1016/j.ijms.2011.06.003
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发表时间：
2012-02-15
影响因子：
1.8
通讯作者：
Goodlett, David R.
中科院分区：
文献类型：
--
作者：
Hengel, Shawna M.;Goodlett, David R.
将回顾使用串联质谱法来识别和表征蛋白质腺苷二磷酸 (ADP) 核糖基化位点。具体来说，我们将重点关注提供肽序列和修饰位点信息的数据采集方案和片段化技术。还讨论了使用合成标准品来帮助表征，以及将 ADP 核糖基化肽转化为核糖基单磷酸化肽的酶法，使鉴定适合传统的磷酸肽表征方法。最后，讨论了这些技术在表征多 ADP-核糖基化位点方面的潜在用途以及固有的挑战。 (C) 2011 Elsevier B.V. 保留所有权利。
The use of tandem mass spectrometry to identify and characterize sites of protein adenosine diphosphate (ADP) ribosylation will be reviewed. Specifically, we will focus on data acquisition schemes and fragmentation techniques that provide peptide sequence and modification site information. Also discussed are uses of synthetic standards to aid characterization, and an enzymatic method that converts ADP-ribosylated peptides into ribosyl mono phosphorylated peptides making identification amenable to traditional phosphopeptide characterization methods. Finally the potential uses of these techniques to characterize poly ADP-ribosylation sites, and inherent challenges, are addressed. (C) 2011 Elsevier B.V. All rights reserved.