Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene.
Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene.
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发表时间：
2001-10
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通讯作者：
D. Pangallo;E. Kaclíková;T. Kuchta;Hana Drahovská
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作者：
D. Pangallo;E. Kaclíková;T. Kuchta;Hana Drahovská
设计了引物，利用聚合酶链反应对编码内毒素的inlB基因特异序列进行检测。在优化的反应条件下，inlB-L/R引物对33株单核增生李斯特菌的敏感性为100%，对其他15株李斯特菌和41株李斯特菌的特异性为100%。这些引物的PCR检出限为10(4)cfu/ml，不受高达10(8)cfu/ml的L. innocua的影响。
Primers were designed for the detection of Listeria monocytogenes by the polymerase chain reaction oriented to specific sequences of the inlB gene encoding an internalin. At optimized reaction conditions, 100% sensitivity (on a panel of 33 strains of L. monocytogenes) and 100% specificity (on panels of 15 strains of other Listeria spp. and 41 other bacteria), were determined for the inlB-L/R primers. The detection limit of PCR with these primers was 10(4) cfu/ml and was not affected by up to 10(8) cfu/ml of L. innocua.