Experimental analysis of the origin of the wing musculature in avian embryos
Experimental analysis of the origin of the wing musculature in avian embryos
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禽类胚胎翅膀肌肉组织起源的实验分析
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
M. Jacob
中科院分区：
文献类型：
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作者：
B. Christ;H. J. Jacob;M. Jacob
体节和部分体细胞板中胚层的种间移植已在鹌鹑和雏鸡胚胎之间进行(阶段12-14H.H.)在未来翅芽的水平上，以考察体节与翅芽肌肉发生的关系。天然鹌鹑核标记的稳定性使其有可能跟踪宿主胚胎中移植的中胚层细胞的发育命运。经过3-7天孵化后的胚胎在翼芽中显示出以下体细胞和体细胞的分布：一旦能够区分中胚层内三个不同细胞密度的区域，体细胞起源的细胞就被限制在由体细胞和体细胞混合组成的可能的肌源性区域，而可能的软骨区以及外胚层下区域只由来自体细胞板状中胚层的细胞组成。进一步孵育后，可见单个肌肉胚泡，也被认为是体细胞和体细胞的混合物。肌束细胞来源于体细胞，而肌肉结缔组织细胞来源于体板中胚层。在体腔腔或在绒毛尿囊膜上移植后，先前从鹌鹑胚胎分离的体板中胚层碎片(阶段14 H.H.)在未来翼芽的水平上，显示出发育良好的骨骼元素，但不能区分任何肌肉组织。这些实验研究支持先前的证据，即翼芽肌源细胞是体细胞起源的。鸡胚臂体节和相邻体板中胚层的组织学和扫描电子显微镜研究(13-15H.H.)结果表明，仍未分化的体细胞在胚胎第14期开始向臂体板中胚层迁移。
SummaryInterspecific grafts of somites, as well as parts of the somatic plate mesoderm, have been made between quail and chick embryos (stages 12–14 H.H.) at the level of the prospective wing bud in order to examine the relationship between somites and wing bud myogenesis. The stability of the natural quail nuclear labelling makes it possible to follow the developmental fate of grafted mesodermal cells in the host embryo. Embryos examined after subsequent incubation periods of 3–7 days show the following distribution of somatic and somitic cells within the wing bud: as soon as the three zones of different cell density within the mesoderm can be distinguished, cells of somitic origin are limited to the prospective myogenic are which is made up of a mixed population of somatic and somitic cells, whereas the prospective chondrogenic area as well as the subectodermal zone only consists of cells originated from the somatic plate mesoderm. After further incubation, single muscle blastema are present which were also seen to be a mixture of somatic and somitic cells. The cells of muscular bundles are of somitic origin, while the muscle connective tissue cells are derived from the somatic plate mesoderm. After grafting into the coelomic cavity or on the chorio-allantoic membrane, fragments of the somatic plate mesoderm previously isolated from quail embryos (stage 14 H.H.) at the level of the prospective wing bud exhibit well developed skeletal elements, but fail to differentiate any musculature. These experimental investigations support previous evidence for a somitic origin of wing bud myogenic cells. Histological and scanning electron microscopic studies of the brachial somites and the adjacent somatic plate mesoderm of chick embryo (stages 13–15 H.H.) reveal that migration of still undifferentiated somitic cells into the brachial somatic plate mesoderm begins to take place in embryos at stage 14.