Inhibition of beta-lactam antibiotics at two different times in the cell cycle of Streptococcus faecium ATCC 9790.
Inhibition of beta-lactam antibiotics at two different times in the cell cycle of Streptococcus faecium ATCC 9790.
复制标题
β-内酰胺抗生素在屎链球菌 ATCC 9790 细胞周期中两个不同时间的抑制作用。
DOI：
10.1128/jb.165.3.682-688.1986
复制
发表时间：
1986
影响因子：
3.2
通讯作者：
Daneo-Moore,L
中科院分区：
文献类型：
--
作者：
Pucci,MJ;Hinks,ET;Dicker,DT;Higgins,ML;Daneo-Moore,L
用亚溶浓度的β-内酰胺类抗生素处理屎链球菌ATCC 9790，发现在细胞分裂周期中有两个不同的分裂周期。一种是由N-甲酰亚胺基硫霉素和甲氧西林诱导的，发生在染色体复制完成之前，另一种是由头孢西丁和头孢菌素诱导的，发生在细胞周期的后期。此外，这些抗生素产生了不同的形态；作用于较早阻滞点的抗生素主要产生“哑铃”，而影响较晚时间的抗生素则形成“柠檬”。N-甲酰亚胺基硫那霉素与头孢西丁联用对该菌株有协同杀灭作用。这些数据表明，β-内酰胺类抗生素在屎沙门氏菌中至少有两个作用部位。
Treatment of Streptococcus faecium ATCC 9790 with sublytic concentrations of beta-lactam antibiotics revealed two different division blocks in the cell division cycle. One block, induced by N-formimidoyl thienamycin and methicillin, occurred before the completion of chromosome replication, whereas the other, induced by cefoxitin and cephalothin, took place later in the cycle. In addition, these antibiotics gave rise to distinct morphological forms; the antibiotics acting at the earlier block point produced mainly "dumbbells," whereas those affecting the later time formed "lemons." When used in combination N-formimidoyl thienamycin and cefoxitin exerted synergistic killing on this strain. These data suggest that beta-lactam antibiotics have at least two sites of action in S. faecium.