ACTIVATED TYPE-I PHOSPHATIDYLINOSITOL KINASE IS ASSOCIATED WITH THE EPIDERMAL GROWTH-FACTOR (EGF) RECEPTOR FOLLOWING EGF STIMULATION
ACTIVATED TYPE-I PHOSPHATIDYLINOSITOL KINASE IS ASSOCIATED WITH THE EPIDERMAL GROWTH-FACTOR (EGF) RECEPTOR FOLLOWING EGF STIMULATION
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DOI：
10.1073/pnas.87.10.3816
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发表时间：
1990-05-01
影响因子：
11.1
通讯作者：
FUJITA, DJ
中科院分区：
文献类型：
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作者：
BJORGE, JD;CHAN, TO;FUJITA, DJ
我们已经表明，I型磷脂酰肌醇（PI）激酶活性与表达人表皮生长因子受体（NRHER5）的小鼠成纤维细胞系中的表皮生长因子（EGF）受体相关，并且在用生理相关浓度的EGF处理细胞后，该活性显著增加。EGF刺激EGF受体免疫沉淀物中EGF受体相关PI激酶活性的时间依赖性增加。加入EGF后15分钟检测到活化，在1和2小时之间达到峰值。在EGF浓度低至10 pM时检测到PI激酶的活化，最大刺激发生在约10 pM。InM。对去酰化PI磷酸产物的分析和非离子依赖性对PI激酶活性的抑制表明，本文所述的PI激酶是I型或PI 3“激酶。这些结果表明EGF对PI型激酶的调节作用，并提示其在EGF受体信号转导途径中的潜在作用。
We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at .apprxeq. 1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic dependent, indicated that the PI kinase described here was type I or PI 3'' kinase. These results demonstrate the regulation of a type PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway.