Selective elevation of the N1-acetylspermidine level in human colorectal adenocarcinomas.
Selective elevation of the N1-acetylspermidine level in human colorectal adenocarcinomas.
复制标题
人结直肠腺癌中 N1-乙酰亚精胺水平的选择性升高。
作者：
S. Takenoshita;S. Matsuzaki;G. Nakano;H. Kimura;H. Hoshi;H. Shoda;T. Nakamura
本研究评估了N1-乙酰亚精胺与人类结直肠腺癌的相关性。游离多胺及其单乙酰化形式的腺癌，腺瘤，和明显健康的粘膜测定使用高效离子交换色谱法。高、中分化腺癌的N1-乙酰亚精胺水平分别为27.30 ± 3.13（S. E.）（n = 99）和22.86 ± 3.60（n = 22）nmol/g湿重。这些值明显高于良性腺瘤（5.38 +/- 0.85 nmol/g，n = 31）和对照粘膜。腺癌的口腔和肛门侧的对照粘膜中的N1-乙酰亚精胺水平分别为5.84 +/- 1.44（n = 57）和7.92 +/- 2.89（n = 50）nmol/g;在对照粘膜和腺瘤之间没有观察到显著差异。三种多胺，腐胺，亚精胺和精胺的平均水平在腺瘤和腺癌的两倍左右，控制粘膜。精脒与精胺的摩尔比在腺瘤和腺癌中均显著高于对照组织。游离多胺浓度与大肠肿瘤恶性程度无明显相关性。这些结果表明，N1-乙酰亚精胺在大肠腺癌的代谢是很大的不同，在腺瘤和非肿瘤性粘膜和N1-乙酰亚精胺可以是一个有前途的生化标志物在人类大肠癌。
The association of N1-acetylspermidine with human colorectal adenocarcinomas has been evaluated in this study. Free polyamines and their monoacetylated forms in adenocarcinomas, adenomas, and apparently healthy mucosae were determined using high-performance ion-exchange chromatography. The N1-acetylspermidine levels in well- and moderately differentiated adenocarcinomas were 27.30 +/- 3.13 (S.E.) (n = 99) and 22.86 +/- 3.60 (n = 22) nmol/g, wet weight, respectively. These values were significantly higher than those of benign adenomas (5.38 +/- 0.85 nmol/g, n = 31) and of control mucosae. The N1-acetylspermidine levels in control mucosae on the oral and anal side of adenocarcinomas were 5.84 +/- 1.44 (n = 57) and 7.92 +/- 2.89 (n = 50) nmol/g, respectively; no significant difference was observed between control mucosae and adenomas. The mean levels of three polyamines, putrescine, spermidine, and spermine in both adenomas and adenocarcinomas were about twice as high as those of control mucosae. The molar ratios of spermidine to spermine were significantly greater in both adenomas and adenocarcinomas than in control tissues. There was no obvious correlation between the free polyamine concentrations and the degree of malignancy of the colorectal tumors. These results suggest that the metabolism of N1-acetylspermidine in colorectal adenocarcinomas is quite different from that in adenomas and in nonneoplastic mucosae and that N1-acetylspermidine can be a promising biochemical marker of cancer in the human large intestine.