Osmoregulation of vasopressin release and gene transcription under acute and chronic hypovolemia in rats
Osmoregulation of vasopressin release and gene transcription under acute and chronic hypovolemia in rats
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DOI：
10.1152/ajpendo.00328.2003
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发表时间：
2004-03-01
影响因子：
5.1
通讯作者：
Oiso, Y
中科院分区：
文献类型：
--
作者：
Kondo, N;Arima, H;Oiso, Y
虽然血浆容量的急性减少被认为是增强精氨酸加压素（AVP）的释放，目前还不清楚是否也有这样的相互作用，在基因转录水平。血浆容量的持续变化如何影响β-内酰胺酶的调节也有待确定。在这项研究中，我们研究了急性和慢性血容量减少如何影响大鼠AVP释放和基因转录的非线性调节。腹腔注射聚乙二醇（PEG）诱导急性低血容量模型，并通过3天禁水（WD）或12天盐负荷（SL）诱导慢性低血容量模型。给大鼠注射等渗或高渗盐水，测定血浆AVP水平和视上核和室旁核AVP杂合（hn）RNA表达，这是基因转录的指标。与各组血浆渗透压摩尔浓度的关系。各组血浆AVP水平与血浆Na水平相关。而回归线与血浆AVP钠之间的控制，WD，SL组几乎相同，血浆钠AVP释放的阈值显着降低，只有在PEG组。AVP hnRNA水平也与对照组和PEG组的血浆Na水平相关，PEG组的阈值显著降低。WD组和SL组AVP hnRNA与血浆Na水平无明显相关性。表明急性和慢性血浆容量减少以不同方式影响AVP释放和基因转录的调控。
Although acute decreases in plasma volume are known to enhance the osmotically induced arginine vasopressin (AVP) release, it is unclear whether there is also such interaction at the level of gene transcription. It also remains to be established how sustained changes in plasma volume affect the osmoregulation. In this study, we examined how acute and chronic decreases in blood volume affected the osmoregulation of AVP release and gene transcription in rats. Acute hypovolemia was induced by intraperitoneal injection of polyethylene glycol (PEG), and chronic hypovolemia was induced by 3 days of water deprivation (WD) or 12 days of salt loading (SL). Rats were injected with isotonic or hypertonic saline, and plasma AVP levels and AVP heteronuclear (hn)RNA expression in the supraoptic and paraventricular nuclei, an indicator of gene transcription. were examined in relation to plasma osmolality in each group. Plasma AVP levels were correlated with plasma Na levels in all groups. Whereas the regression lines relating plasma AVP to Na were almost identical among control, WD, and SL groups, the thresholds of plasma Na for AVP release were significantly decreased only in the PEG group. AVP hnRNA levels were also correlated with plasma Na levels in control and PEG groups, and the thresholds were significantly decreased in the PEG group. In contrast, there was no significant correlation of AVP hnRNA and plasma Na levels in WD and SL groups. Thus it was demonstrated that acute and chronic reduction in plasma volume affected the osmoregulation of AVP release and gene transcription in different ways.