Proteomic analysis of the anti-inflammatory action of minocycline.
Proteomic analysis of the anti-inflammatory action of minocycline.
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DOI：
10.1002/pmic.201000273
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发表时间：
2011-01
期刊：
影响因子：
3.4
通讯作者：
Vernallis, Ann B.
中科院分区：
文献类型：
--
作者：
Dunston, Christopher R.;Griffiths, Helen R.;Lambert, Peter A.;Staddon, Susan;Vernallis, Ann B.
米诺环素具有独立于其抗生素活性的抗炎特性，尽管其潜在的分子机制尚不清楚。米诺环素可降低培养巨噬细胞中脂多糖（LPS）诱导的细胞因子和促炎蛋白的表达。在这里，我们测试了一系列临床上重要的四环素化合物（土霉素、强力霉素、米诺环素和替加环素），结果表明它们都能抑制lps诱导的一氧化氮的产生。我们的新发现是，替加环素比其他四环素化合物更能抑制lps诱导的一氧化氮的产生。为了确定米诺环素的潜在靶点，我们使用2-DE和nanoLC-MS评估了在米诺环素预处理存在或不存在的情况下，LPS诱导的巨噬细胞蛋白质组的变化。我们发现了一些主要参与细胞代谢（ATP合成酶β亚基和醛糖还原酶）或应激反应（热休克蛋白）的蛋白质，它们在LPS的作用下表达改变，其中一些被二甲胺四环素恢复，至少部分恢复。这是第一个记录二甲胺四环素引起的蛋白质组学变化的研究。观察到二甲胺四环素抑制部分（但不是全部）lps诱导的蛋白质组学变化，表明二甲胺四环素特异性地影响一些信号通路，并不能完全抑制巨噬细胞的激活。
Minocycline possesses anti-inflammatory properties independently of its antibiotic activity although the underlying molecular mechanisms are unclear. Lipopolysaccharide (LPS)-induced cytokines and pro-inflammatory protein expression are reduced by minocycline in cultured macrophages. Here, we tested a range of clinically important tetracycline compounds (oxytetracycline, doxycycline, minocycline and tigecycline) and showed that they all inhibited LPS-induced nitric oxide production. We made the novel finding that tigecycline inhibited LPS-induced nitric oxide production to a greater extent than the other tetracycline compounds tested. To identify potential targets for minocycline, we assessed alterations in the macrophage proteome induced by LPS in the presence or absence of a minocycline pre-treatment using 2-DE and nanoLC-MS. We found a number of proteins, mainly involved in cellular metabolism (ATP synthase β-subunit and aldose reductase) or stress response (heat shock proteins), which were altered in expression in response to LPS, some of which were restored, at least in part, by minocycline. This is the first study to document proteomic changes induced by minocycline. The observation that minocycline inhibits some, but not all, of the LPS-induced proteomic changes shows that minocycline specifically affects some signalling pathways and does not completely inhibit macrophage activation.
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影响因子：
6.4
作者：
Chen, BC;Chen, YH;Lin, WW
通讯作者：
Lin, WW
影响因子：
24
作者：
Romero-Perez, Diego;Fricovsky, Eduardo;Yamasaki, Katrina Go;Griffin, Michael;Barraza-Hidalgo, Maraliz;Cillmann, Wolfgang;Villarreal, Francisco
通讯作者：
Villarreal, Francisco
影响因子：
15.9
作者：
Rittner, HL;Hafner, V;Weyand, CM
通讯作者：
Weyand, CM
影响因子：
5
作者：
D'Agostino, P;Arcoleo, F;Cillari, E
通讯作者：
Cillari, E
DOI：
10.1073/pnas.0600554103
发表时间：
2006-06-20
影响因子：
11.1
作者：
Alano, Conrad C.;Kauppinen, Tiina M.;Swanson, Raymond A.
通讯作者：
Swanson, Raymond A.