Freezing response of white bass (Morone chrysops) sperm cells
Freezing response of white bass (Morone chrysops) sperm cells
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DOI：
10.1016/j.cryobiol.2006.01.008
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发表时间：
2006-06-01
期刊：
影响因子：
2.7
通讯作者：
Tiersch, T. R.
中科院分区：
文献类型：
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作者：
Devireddy, R. V.;Campbell, W. T.;Tiersch, T. R.
采用不依赖形状的差示扫描量热仪（DSC）技术，研究了白鲈鱼（WB）精细胞在冷冻过程中的水输运响应。精子悬浮液以20℃/min的冷却速度在两种不同的介质中冷冻：(1)不含冷冻保护剂（CPAs），或(2)含5% （v/v）二甲亚砜（Me2SO）。为了进行计算，将精子细胞建模为长24.8 μ m，直径0.305 μ m的圆柱体，而渗透失活细胞体积（V-b）假设为0.6 V-o，其中V-o为等渗或初始细胞体积。通过水输运模型与实验测定的水输运数据拟合，确定了膜透性参数（参考膜透水性L-pg或L-pg[cpa]）和活化能E-Lp或E-Lp[cpa])的最佳拟合范围为：L-pg = 0.51 ~ 1.7 × 10(-15) m(3)/Ns (0.003 ~ 0.01 μ m/min-atm), E-Lp = 83.6 ~ 131.3 kJ//mol （20.0 ~ 31.4 kcal/mol）。本研究获得的参数表明，菊花精细胞的最佳冷却速率为-22℃/min，与实验确定的最佳冷却速率（类似于16℃/min）非常接近。(c) 2006年Elsevier Inc.出版
The water transport response during freezing of sperm cells of Morone chrysops (white bass, WB) was obtained using a shape-independent differential scanning calorimeter (DSC) technique. Sperm cell suspensions were frozen at a cooling rate of 20 degrees C/min in two different media: (1) without cryoprotective agents (CPAs), or (2) with 5% (v/v) dimethyl sulfoxide (Me2SO). For calculations, the sperm cell was modeled as a cylinder of length 24.8 mu m and diameter of 0.305 mu m, while the osmotically inactive cell volume (V-b) was assumed to be 0.6 V-o, where V-o was the isotonic or the initial cell volume. By fitting a model of water transport to the experimentally determined water transport data, the best fit membrane permeability parameters (reference membrane permeability to water, L-pg or L-pg[cpa] and the activation energy, E-Lp or E-Lp[cpa]) were determined, and ranged from L-pg = 0.51-1.7 x 10(-15) m(3)/Ns (0.003-0.01 mu m/min-atm), and E-Lp = 83.6-131.3 kJ//mol (20.0-31.4 kcal/mol). The parameters obtained in this study suggest that the optimal rate of cooling for M. chrysops sperm cells is -22 degrees C/min, a value that compares closely with experimentally determined optimal rates of cooling (similar to 16 degrees C/min). (c) 2006 Published by Elsevier Inc.