Mutagenesis of outer membrane virulence determinants of the oral spirochete Treponema denticola
Mutagenesis of outer membrane virulence determinants of the oral spirochete Treponema denticola
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DOI：
10.1111/j.1574-6968.1998.tb13047.x
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发表时间：
1998-06-15
影响因子：
2.1
通讯作者：
McBride, BC
中科院分区：
文献类型：
--
作者：
Fenno, JC;Wong, GWK;McBride, BC
齿垢密螺旋体ATCC 35405的成孔主要表面蛋白（Msp）和胰凝乳蛋白酶样蛋白酶（CTLP）与牙周致病性有关。在msp基因的两个位点和CTLP基因座的一个位点构建等位基因置换突变。所有突变株均缺乏Msp六边形阵列外膜超微结构，其中编码CTLP的位点被破坏的菌株CKE不产生CTLP，并且具有异常的Msp表达。msp突变体MHE缺乏Msp，并产生增加水平的CTLP。相反，msp突变体MPE产生少量截短的Msp，但不产生CTLP。Msp和CTLP之间的相互作用在运输或组装的外膜复合物提出，（C）1998年欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
The pore-forming major surface protein (Msp) and the chymotrypsin-like protease (CTLP) of Treponema denticola ATCC 35405 have been implicated in periodontal pathogenicity. Allelic replacement mutations were constructed at two sites in the msp gene and at one site in the CTLP locus. All mutant strains lacked the Msp hexagonal array outer membrane ultrastructure, strain CKE, in which the locus encoding CTLP was disrupted, produced no CTLP and had aberrant Msp expression. The msp mutant MHE lacked Msp, and produced increased levels of CTLP, In contrast, msp mutant MPE made small amounts of a truncated Msp, but produced no CTLP. Interactions between Msp and CTLP in transport or assembly of the outer membrane complex are proposed, (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.