Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics.
Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics.
复制标题
DOI：
10.3390/ijms232415895
复制
发表时间：
2022-12-14
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞功能取决于蛋白质调节复合物在特定细胞位置的动态组装。单分子追踪（SMT）是一种用于体外和体内蛋白质动力学生化表征的首选方法。SMT跟踪活细胞中的单个分子，并提供有关其行为的直接信息。SMT成功应用于哺乳动物模型。然而，哺乳动物细胞提供了一个复杂的环境，其中蛋白质的流动性取决于许多难以通过实验控制的因素。因此，酵母细胞，这是单细胞和充分研究与一个小的和完全测序的基因组，提供了一个有吸引力的替代SMT。组织的简单性、遗传操作的容易性和对基因融合的耐受性都使酵母成为量化主要酶、膜蛋白、核和细胞体的动力学的伟大模型。然而，很少有研究人员将SMT技术应用于酵母。我们的目标是将酵母中的SMT推广到更广泛的研究社区。我们的审查有双重目的。我们解释了SMT是如何在酵母细胞中进行的，我们讨论了酵母SMT的最新见解，同时将它们与高等真核生物的SMT结合起来。
Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes.