Studies on the effect of the hepatocyte-stimulating factor on galactose-beta 1----4-N-acetylglucosamine alpha 2----6-sialyltransferase in cultured hepatocytes.
Studies on the effect of the hepatocyte-stimulating factor on galactose-beta 1----4-N-acetylglucosamine alpha 2----6-sialyltransferase in cultured hepatocytes.
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肝细胞刺激因子对培养肝细胞中半乳糖-β1----4-N-乙酰氨基葡萄糖α2----6-唾液酸转移酶影响的研究。
DOI：
10.1016/0167-4889(86)90087-x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Gospodarek,E
中科院分区：
文献类型：
--
作者：
Woloski,BM;Fuller,GM;Jamieson,JC;Gospodarek,E
大鼠肝脏 Galβ1 → 4GlcNAcα2 → 6 唾液酸转移酶在炎症挑战后以升高的水平释放到血液中：这是称为急性期反应物的血浆蛋白组的典型反应。在本研究中，使用肝实质细胞的原代培养物来证明产生纤维蛋白原等血浆蛋白的相同肝细胞类型也产生和释放唾液酸转移酶。肝脏唾液酸转移酶的产生受到肝脏急性期反应物产生的主要调节因子——肝细胞刺激因子（HSF）的刺激，而另一种单因子白介素-1则不影响肝细胞唾液酸转移酶的产生。唾液酸转移酶的最大增加发生在暴露于 HSF 后 48 小时，这比纤维蛋白原反应晚得多。 HSF 刺激的唾液酸转移酶是 Galβ1 → 4GlcNAcα2 → 6 同工酶。
Rat hepatic Galβ1 → 4GlcNAcα2 → 6 sialyltransferase is released into the blood at elevated levels following an inflammatory challenge: this is a typical response of the group of plasma proteins known as acute-phase reactants. In the present study, primary cultures of liver parenchymal cells are used to demonstrate that the same hepatic cell type that produces plasma proteins such as fibrinogen also produces and releases sialyltransferase. Hepatic production of sialyltransferase is stimulated by a major regulator of hepatic acute-phase reactant production, the hepatocyte-stimulating factor (HSF), while another monokine, interleukin-1, does not affect hepatocyte sialyltransferase production. The maximum increase in sialyltransferase occurs 48 h after exposure to HSF which is considerably later than the fibrinogen response. The sialyltransferase that is stimulated by HSF is the Galβ1 → 4GlcNAcα2 → 6 isozyme.