Isolation of a multigene family containing human alpha-tubulin sequences.
Isolation of a multigene family containing human alpha-tubulin sequences.
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含有人α-微管蛋白序列的多基因家族的分离。
DOI：
10.1016/0022-2836(82)90486-7
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发表时间：
1982
影响因子：
5.6
通讯作者：
Cowan,NJ
中科院分区：
文献类型：
--
作者：
Wilde,CD;Crowther,CE;Cowan,NJ
使用鸡 α-微管蛋白互补 DNA 探针筛选两个人类基因组文库，分离出几个含有共同杂交区域的克隆。克隆片段的限制性图谱显示，虽然每个克隆中保留了大多数位点，但仍存在许多差异，包括含基因区域内的几个差异。这些差异不能仅仅归因于限制性位点多态性，因此反映了密切相关的 α-微管蛋白基因家族的存在。
Screening of two human genomic libraries with a chicken α-tubulin complementary DNA probe has resulted in the isolation of several clones containing a common hybridizing region. Restriction mapping of the cloned fragments reveals that, while the majority of sites are retained in each clone, there are a number of differences, including several within the gene-containing region. These differences cannot be ascribed solely to restriction-site polymorphism, and therefore reflect the existence of a family of closely related α-tubulin genes.