Time-resolved circular dichroism and absorption studies of the photolysis reaction of (carbonmonoxy)myoglobin.
Time-resolved circular dichroism and absorption studies of the photolysis reaction of (carbonmonoxy)myoglobin.
复制标题
（碳单氧基）肌红蛋白光解反应的时间分辨圆二色性和吸收研究。
DOI：
10.1016/s0006-3495(88)83146-1
复制
发表时间：
1988
影响因子：
3.4
通讯作者：
Kliger,DS
中科院分区：
文献类型：
--
作者：
Milder,SJ;Bjorling,SC;Kuntz,ID;Kliger,DS
时间分辨圆二色性（TRCD）和吸收光谱用于跟踪（羰基）肌红蛋白（MbCO）的光解反应。随着CO初始损失的光谱变化，在几百纳秒的时间尺度上观察到Mb产物的可见吸收光谱发生了微妙的变化。在CO损失后，Mb在可见光和近紫外区的CD光谱没有变化。数据表明，在MbCO的配体损失后发现了一种与最终Mb产物结构相似的中间产物。
Time-resolved circular dichroism (TRCD) and absorption spectroscopy are used to follow the photolysis reaction of (carbonmonoxy)myoglobin (MbCO). Following the spectral changes associated with the initial loss of CO, a subtle change is observed in the visible absorption spectrum of the Mb product on a time scale of a few hundred nanoseconds. No changes are seen in the CD spectrum of Mb in the visible and near-UV regions subsequent to the loss of CO. The data suggest the existence of an intermediate found after ligand loss from MbCO that is similar in structure to the final Mb product.