An integrative investigation of the therapeutic mechanism of Ainsliaea fragrans Champ. in cervicitis using liquid chromatography tandem mass spectrometry based on a rat plasma metabolomics strategy
An integrative investigation of the therapeutic mechanism of Ainsliaea fragrans Champ. in cervicitis using liquid chromatography tandem mass spectrometry based on a rat plasma metabolomics strategy
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香茅治疗机制的综合研究。
DOI：
10.1016/j.jpba.2018.04.048
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发表时间：
2018-07-15
影响因子：
3.4
通讯作者：
Feng, Yulin
中科院分区：
文献类型：
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作者：
Zhang, Xiaoyong;He, Mingzhen;Feng, Yulin
宫颈炎是一种非常常见的妇科疾病，可由多种因素引起，如淋病奈瑟菌，沙眼衣原体和生殖支原体感染。宫颈炎长期不愈可导致盆腔炎、输卵管炎、盆腔炎、绒毛膜炎等一系列疾病。然而，宫颈炎的发病机制仍不清楚。杏香兔儿风(AFC)已广泛应用于临床治疗宫颈炎。在本研究中，我们进行了综合调查，包括组织病理学分析和非靶血浆代谢组学分析，在苯酚胶浆诱导的宫颈炎大鼠模型，使用超高效液相色谱-串联四极杆飞行时间质谱法。在综合调查的基础上，使用多变量分析确定了宫颈炎组和对照组之间的代谢组学差异。结果，32个潜在的生物标志物被确定在宫颈炎的反应，并参与花生四烯酸代谢，亚油酸代谢，初级胆汁酸的生物合成，牛磺酸和亚牛磺酸代谢，泛酸和CoA的生物合成，和甘油磷脂代谢。治疗后，与模型组相比，AFC组中共有27种潜在的生物标志物显示出水平改变，12种代谢物包括1-硬脂酰甘油磷酸肌醇、博拉睾酮、lysoPC（16：0）、lysoPC（20：4）、lysoPC（P-16：0）、lysoPC（P-18：0）、lysoPC（P-18：1）、硬脂酰肉毒碱、牛磺酸、lysoPC（17：0）、20-羟基二十碳四烯酸和1-花生四烯酸甘油磷酸肌醇恢复至其正常水平。本研究提示AFC的治疗机制与其内源性代谢产物的改变有关。(C)2018爱思唯尔B. V.保留所有权利。
Cervicitis is an extremely common gynecological disease and can be induced by diverse factors such as Neisseria gonorrhoeae, Chlamydia trachomatis, and Mycoplasma genitalium infections. Long-term unhealed cervicitis may lead to a series of diseases including endometritis, salpingitis, pelvic inflammatory disease, and chorioamnionitis. However, the pathogenesis of cervicitis remains unknown. Ainsliaea fragrans Champ. (AFC) has been widely used in clinical treatment of cervicitis. In the present study, we performed an integrative investigation involving histopathology analysis and non-target plasma metabolomics analysis in a cervicitis rat model induced by phenol mucilage, using ultra-performance liquid chromatography coupled with a tandem quadrupole time-of-flight mass spectrometry approach. Based on the integrative investigation, marked metabolomic differences were identified between the cervicitis and control groups using multivariate analysis. As a result, 32 potential biomarkers were identified in the response to cervicitis, and were involved in arachidonic acid metabolism, linoleic acid metabolism, primary bile acid biosynthesis, taurine and hypotaurine metabolism, pantothenate and CoA biosynthesis, and glycerophospholipid metabolism. After treatment, a total of 27 potential biomarkers exhibited altered levels in the AFC group compared to the model group, and 12 metabolites including 1-stearoylglycerophosphoinositol, bolasterone, lysoPC(16:0), lysoPC(20:4), lysoPC(P-16:0), lysoPC(P-18:0), lysoPC(P-18:1), stearoylcarnitine, taurine, lysoPC(17:0), 20-hydroxyeicosatetraenoic acid, and 1-arachidonoylglycerophosphoinositol returned to their normal levels. This study suggested that the therapeutic mechanism of AFC is related to those altered endogenous metabolites. (C) 2018 Elsevier B.V. All rights reserved.