Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cytosolic calcium
Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cytosolic calcium
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DOI：
10.1016/j.ceca.2005.01.019
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发表时间：
2005-06-01
期刊：
影响因子：
4
通讯作者：
Tschirhart, EJ
中科院分区：
文献类型：
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作者：
Bréchard, S;Bueb, JL;Tschirhart, EJ
在各种刺激下，中性粒细胞释放大量由NADPH氧化酶产生的活性氧（ROS）。这个被称为呼吸爆发的过程依赖于胞质游离钙浓度（[Ca2+](i)）。促炎细胞因子如白细胞介素-8 （IL-8）可能通过启动现象调节ROS的产生。本研究的目的是确定人IL-8对中性粒细胞样二甲沙氧化物分化的HL-60细胞（不等于HL-60细胞）中ROS产生的影响，并进一步研究Ca2+动员在启动过程中的作用。IL-8在10 nM时不产生ROS，但在254 - 136 nM时[Ca2+] i升高。IL-8诱导1 μ M n -甲酰基-l -蛋氨酸-l -亮氨酸-l -苯丙氨酸（fMLF）刺激时ROS释放强烈增强（2倍）。这种增强的ROS生产是依赖于细胞外Ca2+（17.0 +/- 4.5任意单位（A.U.），在Ca2+缺席与56.6 +/- 3.9 A.U.存在1.25 mM的Ca2+）。此外，IL-8增强了fMLF刺激的[Ca2+]增加（375 +/- 35 vs 245 +/- 121 nM, 0.1 μ M fMLF）。1 μ M thapsigargin对非等量HL-60细胞无影响（472 +/- 66 vs 470 +/- 60 nM）。综上所述，Ca2+内流是IL-8充分诱导中性粒细胞启动的必要条件。(c) 2005年Elsevier Ltd出版
In response to a variety of stimuli, neutrophils release large amount of reactive oxygen species (ROS) generated by NADPH oxidase. This process known as the respiratory burst is dependent on cytosolic free calcium concentration ([Ca2+](i)). Proinflammatory cytokines such as interleukin-8 (IL-8) may modulate ROS generation through a priming phenomenon. The aim of this study was to determine the effect of human IL-8 on ROS production in neutrophil-like dimethylsuffoxide-differentiated HL-60 cells (not equal HL-60 cells) and further to examine the role of Ca2+ mobilization during the priming. IL-8 at 10 nM induced no ROS production but a [Ca2+] i rise (254 136 nM). IL-8 induced a strongly enhanced (2 fold) ROS release during stimulation with 1 mu M of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLF). This potentiation of ROS production is dependent of extracellular Ca2+ (17.0 +/- 4.5 arbitrary units (A.U.) in the absence of Ca2+ versus 56.6 +/- 3.9 A.U. in the presence of 1.25 mM of Ca2+). Also, IL-8 enhanced fMLF-stimulated increase in [Ca2+], (375 +/- 35 versus 245 +/- 121 nM, 0.1 mu M of fMLF). IL-8 had no effect on not equal HL-60 cells in response to 1 mu M of thapsigargin (472 +/- 66 versus 470 +/- 60 nM). In conclusion, Ca2+ influx is necessary for a full induction of neutrophil priming by IL-8. (c) 2005 Published by Elsevier Ltd.