Dermal wound healing processes with curcumin incorporated collagen films
Dermal wound healing processes with curcumin incorporated collagen films
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DOI：
10.1016/s0142-9612(03)00625-2
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发表时间：
2004-05-01
期刊：
影响因子：
14
通讯作者：
Jayakumar, R
中科院分区：
文献类型：
--
作者：
Gopinath, D;Ahmed, MR;Jayakumar, R
伤口愈合过程涉及对系统的广泛氧化应激，其通常抑制组织重塑。在本研究中，试图通过从胶原蛋白中缓慢释放抗氧化剂如姜黄素来改善伤口愈合的质量，胶原蛋白也可以作为再生组织的支持基质。姜黄素掺入胶原基质（CICM）治疗组与对照组和胶原治疗组进行比较。生化指标和组织学分析显示，CICM组的伤口缩小，细胞增殖和有效的自由基清除能力增强。当与正常胶原膜相比时，CICM膜的较高收缩温度表明增加的水热稳定性。光谱研究表明，姜黄素结合到胶原蛋白，而不影响其三重螺旋。此外，我们采用2，2 '-偶氮二异丁腈的抗氧化试验来评估CICM的体外抗氧化活性。抗氧化研究表明，CICM更有效地淬灭自由基。本研究为局部应用CICM作为支持皮肤伤口愈合的可行且有效的方法提供了依据。(C)2003爱思唯尔有限公司。保留所有权利。
The wound healing process involves extensive oxidative stress to the system, which generally inhibits tissue remodeling. In the present study, an improvement in the quality of wound healing was attempted by slow delivery of antioxidants like curcumin from collagen, which also acts as a supportive matrix for the regenerative tissue. Curcumin incorporated collagen matrix (CICM) treated groups were compared with control and collagen treated rats. Biochemical parameters and histological analysis revealed that increased wound reduction, enhanced cell proliferation and efficient free radical scavenging in CICM group. The higher shrinkage temperature of CICM films suggests increased hydrothermal stability when compared to normal collagen films. Spectroscopic studies revealed that curcumin was bound to the collagen without affecting its triple helicity. Further we adopted the antioxidant assay using 2,2'-azobisisobutyronitrile to assess in vitro antioxidant activity of CICM. The antioxidant studies indicated that CICM quenches free radicals more efficiently. This study provides a rationale for the topical application of CICM as a feasible and productive approach to support dermal wound healing. (C) 2003 Elsevier Ltd. All rights reserved.