Local renin-angiotensin system in the pineal gland
Local renin-angiotensin system in the pineal gland
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DOI：
10.1016/s0169-328x(97)00339-2
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发表时间：
1998-03-01
期刊：
影响因子：
--
通讯作者：
Bader, M
中科院分区：
文献类型：
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作者：
Baltatu, O;Lippoldt, A;Bader, M
除了经典的内分泌肾素-血管紧张素系统（RAS）外，还描述了脑中的局部RAS。我们试图澄清存在的一个本地RAS在松果体。通过使用核糖核酸酶保护试验，证明可以检测大鼠松果体中血管紧张素原（AOGEN）、血管紧张素受体1A型（AT（1a））和1B型（AT（1b））以及血管紧张素转换酶（ACE）的mRNA。然而，用这种方法不能发现肾素mRNA。通过原位杂交和免疫细胞化学，AOGEN mRNA与星形胶质细胞标志物胶质细胞酸性蛋白共定位。AT（1b）mRNA的表达超过AT（1a）mRNA的表达，并与松果体细胞特异性色氨酸羟化酶共定位。因此，在哺乳动物的松果体中，RAS的组分局部形成。血管紧张素II受体的存在进一步证实了血管紧张素和松果体RAS在该腺体生理学中的作用。(C)1998年Elsevier Science B.V.
Besides the classical endocrine renin-angiotensin system (RAS), a local RAS has been described also in the brain. We attempted to clarify the existence of a local RAS in the pineal gland. Through the use of a ribonuclease protection assay, it proved possible to detect the mRNA for angiotensinogen (AOGEN), for the angiotensin receptor type 1A (AT(1a)) and 1B (AT(1b)) and for the angiotensin-converting enzyme (ACE) in pineal glands from rats. Renin mRNA, however, could not be found by this method. By in situ hybridization and immunocytochemistry, AOGEN mRNA was co-localized with the astrocyte marker glial fibrillary acidic protein. AT(1b) mRNA expression exceeded the expression of AT(1a) mRNA and was co-localized with the pinealocyte-specific tryptophan hydroxylase. Thus, in the mammalian pineal gland there is a local formation of the components of the RAS. The presence of angiotensin II receptors further substantiates a role for angiotensins and the pineal RAS in the physiology of this gland. (C) 1998 Elsevier Science B.V.