Identification of genes for mycothiol biosynthesis in Streptomyces coelicolor A3(2).
Identification of genes for mycothiol biosynthesis in Streptomyces coelicolor A3(2).
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发表时间：
2006-02
影响因子：
3
通讯作者：
Joo‐Hong Park;C. Cha;J. Roe
中科院分区：
文献类型：
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作者：
Joo‐Hong Park;C. Cha;J. Roe
菌硫醇是一种由多种放线菌产生的低分子量硫醇化合物，已被认为具有抗氧化和解毒作用。为了研究天蓝色链霉菌中分枝硫醇的代谢和作用，根据与分枝杆菌基因的序列同源性预测了生物合成基因（mshA、B、C和D），并通过实验进行了证实。通过 PCR 靶向诱变破坏 mshA、C 和 D 基因，导致菌硫醇无法合成，而 mshB 突变将其水平降低至野生型的约 10%。结果表明，mshA、C 和 D 基因编码非冗余生物合成酶，而 MshB（乙酰化酶）的酶活性由至少一种其他基因产物共享，最有可能是 mca 基因产物（酰胺酶）。
Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and D) were predicted based on sequence homology with the mycobacterial genes and confirmed experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about 10% of the wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene product, most likely the mca gene product (amidase).