The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs
The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs
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DOI：
10.3233/bir-2008-0473
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发表时间：
2008-01-01
期刊：
影响因子：
1.1
通讯作者：
Bader, D. A.
中科院分区：
文献类型：
--
作者：
Akanji, O. O.;Lee, D. A.;Bader, D. A.
在关节软骨中检测到内源性电活动。先前已经提出，相关的电流和电位对软骨中的机械转导过程是重要的。本研究利用已建立的三维软骨细胞琼脂糖模型系统，研究了直流电对细胞增殖和基质合成的影响，将从掌指关节分离的牛软骨细胞接种在琼脂糖结构中，并暴露于4 mA/cm（2）的电流密度下6 h，该电流强度和时间显示可以维持细胞活力。通过蛋白质掺入和mRNA测量，分别使用放射性标记和实时定量PCR，结果表明，电流对蛋白质合成，细胞增殖和mRNA表达水平没有系统的影响。这些数据表明，刺激模式和模型系统对于软骨细胞代谢的体外调节都是至关重要的。
Endogenous electrical activity has been detected in articular cartilage. It has previously been suggested that the associated electrical currents and potentials are important to the mechanotransduction processes in cartilage. The present study investigates the effects of direct current on cell proliferation and matrix synthesis, using the well established 3D chondrocyte agarose model system.Bovine chondrocytes isolated from metacarpalphalangeal joints were seeded in agarose constructs and exposed to a current density of 4 mA/cm(2) for 6 h, a magnitude and period which was shown to maintain cell viability. The influence of the optimized electric stimulus was assessed by protein incorporation and mRNA measurements, using radiolabels and real-time QPCR, respectively.Results indicated no systematic influences of electrical current on protein synthesis, cell proliferation and mRNA expression levels. These data suggest that both the mode of stimulation and the model system are critical for the in vitro modulation of chondrocyte metabolism.