Infrared spectroscopy as a novel tool to diagnose onychomycosis
Infrared spectroscopy as a novel tool to diagnose onychomycosis
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DOI：
10.1111/bjd.17199
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发表时间：
2019-03-01
影响因子：
10.3
通讯作者：
Delanghe, J.
中科院分区：
文献类型：
--
作者：
De Bruyne, Sander;Speeckaert, R.;Delanghe, J.
背景甲真菌病病原菌的检测方法尚不完善。仍然需要一种廉价、快速和易于执行的诊断工具，具有高能力来区分生物体。目的探讨衰减全反射傅里叶变换红外光谱（ATR-FTIR）在培养、离体和体内指甲模型中是否能检测和区分病原体。方法进行方法学研究。体外甲模型和体内试验均在某高校附属医院进行。结果对培养的真菌进行分析，发现皮肤真菌（1692-1606和1044-1004 cm(-1)）与非皮肤真菌和酵母（973-937 cm(-1)）的光谱差异，通过树形图证实了不同属或种样品之间的良好分离。对生长在离体指甲上的皮肤真菌、非皮肤真菌和酵母菌的研究发现，从1200厘米到900厘米（-1）存在显著差异。预测模型对未感染指甲、皮肤真菌感染指甲、非皮肤真菌感染指甲和酵母感染指甲的分类准确率为969%。对皮肤菌属、非皮肤菌属和酵母菌属或种的分类正确率分别为910%、977%和986%。体内感染和未感染指甲的光谱也显示出明显的光谱差异（3000-2811 cm（-1）、1043-950 cm（-1）和1676-1553 cm(-1)），这可以通过树突图上的两个主要簇（未感染与感染）来说明。结论ATR-FTIR光谱法可能是一种有前途的、快速和准确的测定甲真菌病的方法，包括鉴定病原菌，而不需要长时间的真菌培养。
BackgroundThe determination of causative organisms of onychomycosis is still not optimal. There remains a need for a cheap, fast and easy-to-perform diagnostic tool with a high capacity to distinguish between organisms.ObjectivesTo determine whether attenuated total-reflectance Fourier transform infrared (ATR-FTIR) spectroscopy can detect and differentiate causative agents in culture-based, ex vivo nail and in vivo nail models.MethodsA methodological study was conducted. Both the ex vivo nail model and in vivo pilot study were carried out in an academic university hospital.ResultsAnalysis of cultured fungi revealed spectral differences for dermatophytes (1692-1606 and 1044-1004 cm(-1)) and nondermatophytes and yeasts (973-937 cm(-1)), confirmed by dendrograms showing an excellent separation between samples from different genera or species. Exploration of dermatophytes, nondermatophytes and yeasts growing on ex vivo nails exposed prominent differences from 1200 to 900 cm(-1). Prediction models resulted in a 969% accurate classification of uninfected nails and nails infected with dermatophytes, nondermatophytes and yeasts. Overall correct classification rates of 910%, 977% and 986% were obtained for discrimination between dermatophyte, nondermatophyte and yeast genera or species, respectively. Spectra of in vivo infected and uninfected nails also revealed distinct spectral differences (3000-2811 cm(-1), 1043-950 cm(-1) and 1676-1553 cm(-1)), illustrated by two main clusters (uninfected vs. infected) on a dendrogram.ConclusionsOur data suggest that ATR-FTIR spectroscopy may be a promising, fast and accurate method to determine onychomycosis, including identification of the causative organism, bypassing the need for lengthy fungal cultures.