High Serum Arginase I Levels in Asthma: its Correlation with High-Sensitivity C-Reactive Protein
High Serum Arginase I Levels in Asthma: its Correlation with High-Sensitivity C-Reactive Protein
复制标题
DOI：
10.3109/02770903.2010.528496
复制
发表时间：
2011-02-01
影响因子：
1.9
通讯作者：
Oka, Mikio
中科院分区：
文献类型：
--
作者：
Ogino, Keiki;Obase, Yasushi;Oka, Mikio
背景哮喘小鼠肺组织中β-淀粉酶I的诱导作用已引起人们的广泛关注。然而，以往的研究对支气管哮喘患者血清中β-淀粉酶活性的变化并不一致。目标.本研究的目的是评估血清中的乙酰胆碱酯酶I在哮喘患者中的临床意义。方法.采用酶联免疫吸附试验（ELISA）测定了23例非吸烟哮喘患者和30例健康人血清中的α-淀粉酶Ⅰ（α-淀粉酶Ⅰ）水平，并分析了其与临床指标的相关性。结果与健康对照组（27.2 +/- 12.9 ng/mL）相比，哮喘患者（平均值+/- SD 67.4 +/- 41.0 ng/mL）血清中的乙酰胆碱酯酶I水平显著升高。在健康对照组中，不同性别组之间没有观察到辅酶I水平的差异，但在不同年龄组之间观察到差异。在哮喘患者中，不同年龄、性别和吸入性类固醇治疗组之间的血清β-淀粉酶I水平无差异，但不同特应性状态组之间的血清β-淀粉酶I水平有差异。非特应性哮喘患者显示显着较高的血清乙酰胆碱酯酶I水平与特应性哮喘患者和健康对照组相比，虽然没有观察到差异特应性哮喘患者和健康对照组之间。斯皮尔曼相关分析显示，健康对照组血清谷丙转氨酶I水平与年龄呈显著负相关，与红细胞数呈正相关;哮喘组血清谷丙转氨酶I水平与丙氨酸氨基转移酶（ALT）、超敏C反应蛋白（hs-CRP）呈显著正相关，与免疫球蛋白E（IgE）呈显著负相关。结论.哮喘患者血清中高水平的乙酰胆碱酯酶I可能与非特应性哮喘的气道炎症有关。
Background. Much attention has been directed to the induction of arginase I in the lung of asthmatic mice. However, there is no agreement on the changes of serum arginase activity in asthmatic patients among previous studies. Objectives. The aim of this study was to evaluate the clinical relevance of serum arginase I in asthmatic patients. Methods. Serum arginase I was examined cross-sectionally in non-smoking asthmatic patients (n = 23) and healthy individuals (n = 30) using enzyme-linked immunosorbent assay ( ELISA) and its correlations with several clinical parameters were investigated. Results. Serum levels of arginase I were significantly increased in asthmatic patients ( mean +/- SD 67.4 +/- 41.0 ng/mL) compared with healthy controls ( 27.2 +/- 12.9 ng/mL). In healthy controls, a difference in arginase I levels was not observed between sex groups but was observed between age groups. In asthmatic patients, serum arginase I levels were not different between groups of age, sex, and inhalation steroid therapy but were different between groups of atopic status. Non-atopic asthmatic patients revealed significantly high serum arginase I levels compared with atopic asthmatic patients and healthy controls although no difference was observed between atopic asthmatic patients and healthy controls. Spearman's correlation analysis showed that serum arginase I level had a significant negative correlation with age and a positive correlation with red blood cell numbers in healthy controls, whereas in asthmatic patients, it had significant positive correlations with alanine aminotransferase (ALT), high-sensitivity C-reactive protein (hs-CRP) and a negative correlation with immunoglobulin-E (IgE). Conclusions. High serum arginase I levels in asthmatic patients may be associated with airway inflammation in non-atopic asthma.