A screen for genetic loci required for body-wall muscle development during embryogenesis in Caenorhabditis elegans.
A screen for genetic loci required for body-wall muscle development during embryogenesis in Caenorhabditis elegans.
复制标题
秀丽隐杆线虫胚胎发生过程中体壁肌肉发育所需遗传位点的筛选。
DOI：
10.1093/genetics/137.2.483
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Fire,A
中科院分区：
文献类型：
--
作者：
Ahnn,J;Fire,A
我们已经使用了可用的染色体缺陷筛选的遗传位点，其合子的表达是必需的体壁肌细胞在胚胎发生过程中秀丽隐杆线虫。为了测试肌肉细胞分化，我们测定了收缩功能和肌肉特异性结构蛋白的表达。针对两种肌球蛋白重链亚型（unc-54和myo-3基因的产物）的单克隆抗体用于检测体壁肌肉分化。我们已经筛选了77个缺陷，覆盖了大约72%的基因组。缺乏纯合子在大多数情况下染色抗体体壁肌球蛋白，并在许多情况下观察到肌肉收缩功能。我们已经确定了两个区域显示肌球蛋白重链基因表达的明显缺陷。去除染色体V的左尖端的缺陷的纯合子胚胎不能积累myo-3和unc-54产物，但表达下皮、咽和神经发育的特征性抗原。胚胎缺乏一个大区域的染色体III积累的unc-54产品，但不是myo-3产品。我们的结论是，只有少数位点的合子表达是唯一需要通过一个肌肉细胞的命运。
We have used available chromosomal deficiencies to screen for genetic loci whose zygotic expression is required for formation of body-wall muscle cells during embryogenesis in Caenorhabditis elegans. To test for muscle cell differentiation we have assayed for both contractile function and the expression of muscle-specific structural proteins. Monoclonal antibodies directed against two myosin heavy chain isoforms, the products of the unc-54 and myo-3 genes, were used to detect body-wall muscle differentiation. We have screened 77 deficiencies, covering approximately 72% of the genome. Deficiency homozygotes in most cases stain with antibodies to the body-wall muscle myosins and in many cases muscle contractile function is observed. We have identified two regions showing distinct defects in myosin heavy chain gene expression. Embryos homozygous for deficiencies removing the left tip of chromosome V fail to accumulate the myo-3 and unc-54 products, but express antigens characteristic of hypodermal, pharyngeal and neural development. Embryos lacking a large region on chromosome III accumulate the unc-54 product but not the myo-3 product. We conclude that there exist only a small number of loci whose zygotic expression is uniquely required for adoption of a muscle cell fate.