Rapid genome sequencing of RNA viruses

Rapid genome sequencing of RNA viruses
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DOI:
10.3201/eid1302.061032
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发表时间:
2007-02-01
影响因子:
11.8
通讯作者:
Nishimura, Hidekazu
Nishimura, Hidekazu
中科院分区:
医学2区
文献类型:
--
作者:
Mizutani, Tetsuya;Endoh, Daiji;Nishimura, Hidekazu

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我们开发了一种用于快速测定病毒RNA序列的系统,由此从培养的病毒分离株获得基因组序列而无需亚克隆到质粒载体中。这种方法为应对未知或无法分型的新兴病毒的挑战提供了新的机会。
We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.