High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment
High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment
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DOI：
10.4161/org.25425
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发表时间：
2013-07-01
期刊：
影响因子：
2.3
通讯作者：
Yim, Evelyn K. F.
中科院分区：
文献类型：
--
作者：
Ankam, Soneela;Teo, Benjamin K. K.;Yim, Evelyn K. F.
体内的干细胞被安置在称为干细胞龛的功能微环境中。由于小生境组分可以调节干细胞的行为，如增殖，迁移和分化，因此评估这些组分对于确定其维持或分化的最佳平台至关重要。在这篇综述中，我们讨论了有助于干细胞研究的高通量筛选试验的发展的方法和技术，包括使能技术，如成熟的多孔/微孔板和机器人点样，以及新兴技术，如微流体，微接触印刷和光刻。我们还讨论了利用高通量筛选平台研究干细胞对细胞外基质、地形、生物材料和干细胞龛中刚度梯度的反应的研究。上述技术的结合可以为进一步发展高通量技术和干细胞研究的新前景奠定基础。
Stem cells in vivo are housed within a functional microenvironment termed the stem cell niche. As the niche components can modulate stem cell behaviors like proliferation, migration and differentiation, evaluating these components would be important to determine the most optimal platform for their maintenance or differentiation. In this review, we have discussed methods and technologies that have aided in the development of high throughput screening assays for stem cell research, including enabling technologies such as the well-established multiwell/microwell plates and robotic spotting, and emerging technologies like microfluidics, micro-contact printing and lithography. We also discuss the studies that utilized high throughput screening platform to investigate stem cell response to extracellular matrix, topography, biomaterials and stiffness gradients in the stem cell niche. The combination of the aforementioned techniques could lay the foundation for new perspectives in further development of high throughput technology and stem cell research.