Vaccines containing de-N-acetyl sialic acid elicit antibodies protective against neisseria meningitidis groups B and C.
Vaccines containing de-N-acetyl sialic acid elicit antibodies protective against neisseria meningitidis groups B and C.
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DOI：
10.4049/jimmunol.0803677
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发表时间：
2009-05-15
期刊：
影响因子：
--
通讯作者：
Flitter BA
中科院分区：
文献类型：
--
作者：
Moe GR;Bhandari TS;Flitter BA
Murine monoclonal antibodies (mAbs) that were produced by immunization with a vaccine containing the N-propionyl derivative of Neisseria meningitidis group B (MenB) capsular polysaccharide (NPr MBPS) mediate protective responses against MenB but were not reactive with unmodified MBPS or chemically identical human polysialic acid (PSA). Recently, we showed that some of the mAbs were reactive with MBPS derivatives that contain de-N-acetyl sialic acid residues (Moe et al. 2005, Infect Immun 73:2123–2128). In this study we evaluated the immunogenicity of de-N-acetyl sialic acid-containing derivatives of PSA (de-N-acetyl PSA) in mice. Four de-N-acetyl PSA antigens were prepared and conjugated to tetanus toxoid, including completely de-N-acetylated PSA. All of the vaccines elicited anti-de-N-acetyl PSA responses (titers ≥1:10,000) but only vaccines enriched for non-reducing end de-N-acetyl residues by treatment with exoneuraminidase or complete de-N-acetylation elicited high titers against the homologous antigen. Also, non-reducing end de-N-acetyl residue-enriched vaccines elicited IgM and IgG antibodies of all subclasses that could bind to MenB. The results suggest that the zwitterionic characteristic of neuraminic acid, particularly at the non-reducing end may be important for processing and presentation mechanisms that stimulate T cells. Antibodies elicited by all four vaccines were able to activate deposition of human complement proteins and passively protect against challenge by MenB in the infant rat model of meningococcal bacteremia. Some vaccine antisera mediated bactericidal activity against a MenC strain with human complement. Thus, de-N-acetyl PSA antigens are immunogenic and elicit antibodies that can be protective against MenB and C strains.
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影响因子：
4.4
作者：
Gallorini, Simona;Berti, Francesco;Wack, Andreas
通讯作者：
Wack, Andreas
影响因子：
64.5
作者：
Cobb, BA;Wang, O;Kasper, DL
通讯作者：
Kasper, DL
DOI：
10.1084/jem.185.11.1929
发表时间：
1997-06-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Pon RA;Lussier M;Yang QL;Jennings HJ
通讯作者：
Jennings HJ
影响因子：
3.1
作者：
Coquillat, D;Bruge, J;Rougon, G
通讯作者：
Rougon, G
影响因子：
3.6
作者：
Moe, GR;Dave, A;Granoff, DM
通讯作者：
Granoff, DM