Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.
Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.
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DOI：
10.4137/grsb.s32520
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Fujii H
中科院分区：
文献类型：
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作者：
Fujita T;Fujii H
为了分离保留其分子相互作用的特定基因组区域，允许直接鉴定染色质结合分子，我们开发了两种位点特异性染色质免疫沉淀（位点特异性ChIP）技术，插入ChIP（iChIP）和工程化DNA结合分子介导的ChIP（enChIP），使用成簇规则间隔短回文重复序列（CRISPR）系统或转录激活因子样（TAL）蛋白。基本上，基因座特异性ChIP由基因座标记和亲和纯化组成，并且可以与下游分析相结合以鉴定与靶基因组区域相关的分子。本文综述了基因座特异性ChIP在基因组功能分析中的应用，包括转录和表观遗传调控。
To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP) using the clustered regularly interspaced short palindromic repeats (CRISPR) system or transcription activator-like (TAL) proteins. Essentially, a locus-specific ChIP consists of locus-tagging and affinity purification and can be combined with downstream analyses to identify molecules associated with the target genomic regions. In this review, we discuss the applications of locus-specific ChIP to analyze the genome functions, including transcription and epigenetic regulation.