Structure, chromosomal localization, and expression analysis of the mouse inhibin/activin beta C (Inhbc) gene.
Structure, chromosomal localization, and expression analysis of the mouse inhibin/activin beta C (Inhbc) gene.
复制标题
小鼠抑制素/激活素 β C (Inhbc) 基因的结构、染色体定位和表达分析。
作者：
J. Schmitt;G. Hötten;N. Jenkins;D. Gilbert;N. Copeland;J. Pohl;H. Schrewe
小鼠抑制素/激活素βC基因(Inhbc)是转化生长因子-β(TGF-β)超家族成员之一，已被克隆、定位和鉴定。该基因全长约14kb，由两个外显子组成，定位于小鼠10号染色体的远端区域，该区域与人类抑制素/激活素βC基因(INHBC)定位在12q13.1染色体上。主要的翻译产物是一个352个氨基酸的前蛋白。其成熟的C末端结构域由116个氨基酸组成，与人类同源物有94%的同源性。引物延伸分析表明，转录开始于翻译起始点的上游约130bp，启动子中没有发现TATA盒。核糖核酸酶保护分析表明，小鼠Inhbc主要在成人肝脏中表达。胚胎的表达从怀孕14.5天开始检测到。
The mouse inhibin/activin beta C gene (Inhbc), a member of the transforming growth factor-beta (TGF-beta) superfamily, was cloned, mapped, and characterized. The gene spans approximately 14 kb, is composed of two exons, and maps to the distal region of mouse chromosome 10, which is syntenic to chromosome 12q13.1, where the human inhibin/activin beta C gene (INHBC) maps. The primary translation product is a preproprotein of 352 amino acids. The mature C-terminal domain of 116 amino acids shares 94% identity with its human homolog. Primer extension analysis shows that transcription starts approximately 130 bp upstream of the translation initiation site, and no TATA box was found in the promoter. Ribonuclease protection analyses reveal that mouse Inhbc is predominantly expressed in adult liver. Embryonic expression is detected beginning from Day 14.5 of gestation.