IDENTIFICATION OF 3 GENES NONESSENTIAL FOR GROWTH IN CELL-CULTURE NEAR THE RIGHT TERMINUS OF THE UNIQUE SEQUENCES OF LONG COMPONENT OF HERPES-SIMPLEX VIRUS-1
IDENTIFICATION OF 3 GENES NONESSENTIAL FOR GROWTH IN CELL-CULTURE NEAR THE RIGHT TERMINUS OF THE UNIQUE SEQUENCES OF LONG COMPONENT OF HERPES-SIMPLEX VIRUS-1
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DOI：
10.1016/0042-6822(90)90534-x
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发表时间：
1990-08-01
期刊：
影响因子：
3.7
通讯作者：
ROIZMAN, B
中科院分区：
文献类型：
--
作者：
BARKER, DE;ROIZMAN, B
我们报告了单纯疱疹病毒1重组体的构建，其中四个开放阅读框的全部或部分编码序列已被删除。在重组体 R7101 和 R7108 中，含有 dUTPase 基因编码序列和晚期基因启动子调控结构域的 BamHI D'' 片段分别被含有胸苷激酶基因的片段替换或删除。除了 BamHI D'' 之外，重组 R7107 还缺少相邻开放阅读框 UL51 3'' 端的总共 244 个预测氨基酸中的 202 个。重组R7105中的缺失包含两个基因，即整个开放阅读框UL47和UL46的氨基末端。这些基因映射到指定 .alpha 的旁边。反式诱导因子。 R7105、R7101 和 R7108 在病毒复制方面未表现出明显的缺陷。重组 R7107 形成微小噬斑，并在亚汇合培养物中的增殖细胞中复制得最好。结果表明，增殖细胞至少提供由 UL51 编码的蛋白质表达的部分功能。
We report the construction of herpes simplex virus 1 recombinants from which all or part of the coding sequences of four open reading frames have been deleted. In recombinants R7101 and R7108, the BamHI D'' fragment containing the coding sequences of the dUTPase gene and the promoter-regulatory domain of a late gene was either replaced with a fragment containing a thymidine kinase gene or deleted, respectively. The recombinant R7107 lacks, in addition to BamHI D'', 202 of a total of 244 predicted amino acids from the 3'' end of the adjacent open reading frame UL51. The deletion in recombinant R7105 encompasses two genes, i.e., the entire open reading frame UL47 and the amino terminus of UL46. These genes map Next to that specifying the .alpha. trans-inducing factor. R7105, R7101, and R7108 do not exhibit demonstrable defects in viral replication. The recombinant R7107 forms minute plaques and replicates best in multiplying cells in subconfluent cultures. The results indicate that the multiplying cells supply at least some of the functions expressed by the protein encoded in UL51.