Comparative iTRAQ-based proteomic analysis provides insight into a complex regulatory network of Pogostemon cablin in response to exogenous MeJA and Ethrel
Comparative iTRAQ-based proteomic analysis provides insight into a complex regulatory network of Pogostemon cablin in response to exogenous MeJA and Ethrel
复制标题
基于 iTRAQ 的比较蛋白质组学分析可深入了解广藿酮 cablin 响应外源 MeJA 和 Ethrel 的复杂调控网络
DOI：
10.1016/j.indcrop.2019.111661
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发表时间：
2019-11-15
影响因子：
5.9
通讯作者：
Chen, Likai
中科院分区：
文献类型：
--
作者：
Li, Junren;Chen, Xiuzhen;Chen, Likai
外源激素的应用是调控经济植物生产和高价值天然医药产品合成的有效可行的方法。茉莉酸和乙烯是调控广藿香生长发育、次生代谢和逆境响应的两种重要植物激素。然而，这些植物激素在这种重要药用植物中的调节机制迄今尚未阐明。本研究探讨了外源茉莉酸甲酯（MeJA）、乙threl （ETH）以及MeJA和ETH同时处理对茉莉叶片次生代谢物和光合作用的影响。为了揭示参与激素诱导机制的潜在蛋白质功能网络，我们使用相对和绝对定量等压标签（iTRAQ）对不同处理的叶片进行了蛋白质组学分析。在三种不同处理前后，分别鉴定出254、229和400种参与多种生理过程的差异表达蛋白（DEPs）。通过平行反应监测（PRM）和qRT-PCR进一步验证定量数据。功能富集分析表明，MeJA处理后，光合作用相关蛋白的表达量显著增加，而次级代谢物生物合成和硫胺素代谢相关蛋白的表达量显著下调。外源ETH主要抑制叶片碳水化合物和能量代谢相关蛋白的表达，而显著提高光合作用蛋白丰度和内质网蛋白加工。MeJA和ETH处理后，与碳水化合物和能量代谢、次生代谢物生物合成、酪氨酸代谢、硫胺素代谢和脂质代谢相关的蛋白质表达均显著下调，而与光合作用、苯丙类生物合成和氮代谢相关的蛋白质表达均显著上调。本研究首次采用基于itraq的蛋白质组学方法，全面阐明了白藜芦醇的外源激素应答，提供了参与次生代谢调控的重要途径/蛋白，为药用成分的遗传改良提供了可能。
Exogenous hormone application is an effective and feasible method to regulate production of economic plant and synthesis of high-value natural medical products. Jasmonate and ethylene are two crucial plant hormones regulate growth and development, secondary metabolism, and stress responses in Pogostemon cablin. However, the regulatory mechanism of these phytohormones in this important medicinal plant has not been elucidated to date. In the present study, alterations of secondary metabolites and photosynthesis of P. cablin leaves treated with exogenous methyl jasmonate (MeJA), ethrel (ETH), and simultaneously with both MeJA and ETH, respectively, were explored. To uncover the potential protein functional network involved in the hormone-induction mechanism, we performed a proteomic analysis of differently treated leaves using isobaric tags for relative and absolute quantification (iTRAQ). In P. cablin leaves before and after three different treatments, 254, 229 and 400 differentially expressed proteins (DEPs) involving a wide range of physiological processes were clearly identified respectively. The quantitative data were further validated by parallel reaction monitoring (PRM) and qRT-PCR. Functional enrichment analysis indicated photosynthesis-related proteins showed a significant increase expression in P. cablin leaves after MeJA treatment, while proteins involved in secondary metabolite biosynthesis and thiamine metabolism were significantly down-regulated. Exogenous ETH mainly repressed the expression of proteins related to carbohydrate and energy metabolism in P. cablin leaves while markedly raised protein abundance in photosynthesis and protein processing in the endoplasmic reticulum. Regarding P. cablin leaves treated with both MeJA and ETH, expression of proteins related to carbohydrate and energy metabolism, secondary metabolite biosynthesis, tyrosine metabolism, thiamine metabolism and lipid metabolism were significantly down-regulated, while that of proteins involved in photosynthesis, phenylpropanoid biosynthesis and nitrogen metabolism were considerably up-regulated. This is the first study using iTRAQ-based proteomics approach to comprehensively elucidate exogenous hormone response of P. cablin, providing important pathways/proteins involved in secondary metabolism regulation, which will contribute to potential genetic improvement of medical constituents.