Identification of Plasma hsa_circ_0005397 and Combined With Serum AFP, AFP-L3 as Potential Biomarkers for Hepatocellular Carcinoma.
Identification of Plasma hsa_circ_0005397 and Combined With Serum AFP, AFP-L3 as Potential Biomarkers for Hepatocellular Carcinoma.
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血浆 hsa_circ_0005397 的鉴定并与血清 AFP、AFP-L3 联合作为肝细胞癌的潜在生物标志物
DOI：
10.3389/fphar.2021.639963
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发表时间：
2021
影响因子：
5.6
通讯作者：
Wang F
中科院分区：
文献类型：
--
作者：
Liu R;Li Y;Wu A;Kong M;Ding W;Hu Z;Chen L;Cai W;Wang F
背景：越来越多的证据表明，环状RNA（circRNA）在肝细胞癌（HCC）的发生和发展中发挥着至关重要的作用。然而，血浆circRNA在HCC中的表达模式和临床应用价值仍然很大程度上未知。在此，我们探讨了血浆 hsa_circ_0005397 在 HCC 诊断和预后中的作用。方法：采用实时定量聚合酶链式反应（qRT-PCR）检测血浆hsa_circ_0005397的表达水平。通过RNase R测定、Sanger测序和HCC细胞培养证实血浆hsa_circ_0005397的鉴定和来源。此外，通过受试者工作特征（ROC）曲线评估其诊断价值，并通过动态监测和Kaplan-Meier曲线分析评估HCC患者的预后价值。结果：HCC患者血浆hsa_circ_0005397的表达高于良性肝病患者和健康对照（均p < 0.05）。此外，它与HCC患者的肿瘤大小（p = 0.020）和TNM分期（p = 0.006）密切相关。血浆 hsa_circ_0005397 的 ROC 曲线下面积为 0.737，95% 置信区间为 0.671–0.795。此外，血浆hsa_cic_0005397、血清AFP和AFP-L3的组合可以提高HCC的诊断敏感性。此外，动态监测血浆 hsa_cic_0005397 可能有助于我们预测 HCC 患者手术切除后的复发或转移。此外，血浆hsa_cic_0005397的增加与HCC患者总生存期缩短密切相关（p = 0.007）。结论：血浆 has_circ_0005397 代表了 HCC 的一种新型非侵入性生物标志物。此外，血浆hsa_cic_0005397、血清AFP和AFP-L3的组合可能会提高HCC的诊断价值。
Background: Mounting evidence has demonstrated that circular RNA (circRNA) plays crucial roles in the occurrence and development of hepatocellular carcinoma (HCC). However, the expression pattern and clinical application value of plasma circRNA in HCC are still largely unknown. Herein, we explored the role of plasma hsa_circ_0005397 in diagnosis and prognosis of HCC. Methods: The expression level of plasma hsa_circ_0005397 was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The identification and origin of plasma hsa_circ_0005397 were confirmed by RNase R assay, Sanger sequencing and HCC cell culture. In addition, its diagnostic value was assessed by receiver operating characteristic (ROC) curve and prognostic value was evaluated by dynamics monitoring and Kaplan–Meier curve analyses in HCC patients. Results: The expression of plasma hsa_circ_0005397 was higher in patients with HCC than that in patients with benign liver diseases and healthy controls (both p < 0.05). Moreover, it was closely correlated with tumor size (p = 0.020) and TNM stage (p = 0.006) of HCC patients. The area under the ROC curve of plasma hsa_circ_0005397 was 0.737 and 95% confidence interval was 0.671–0.795. Furthermore, the combination of plasma hsa_cic_0005397, serum AFP and AFP-L3 could improve the diagnostic sensitivity of HCC. Additionally, dynamic monitoring plasma hsa_cic_0005397 might help us predict recurrence or metastasis in HCC patients after surgical resection. Besides, the increased plasma hsa_cic_0005397 was closely correlated with shorter overall survival of HCC patients (p = 0.007). Conclusion: Plasma has_circ_0005397 represents a novel noninvasive biomarker for HCC. Moreover, the combination of plasma hsa_cic_0005397, serum AFP and AFP-L3 might improve the diagnostic value for HCC.
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