Implementation of data-dependent acquisitions in the study of melphalan DNA adducts by miniaturized liquid chromatography coupled to electrospray tandem mass spectrometry
Implementation of data-dependent acquisitions in the study of melphalan DNA adducts by miniaturized liquid chromatography coupled to electrospray tandem mass spectrometry
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DOI：
10.1002/rcm.1578
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发表时间：
2004-01-01
影响因子：
2
通讯作者：
Esmans, EL
中科院分区：
文献类型：
--
作者：
Van den Driessche, B;Van Dongen, W;Esmans, EL
由外源物质引起的 DNA 缺陷的研究，特别是 DNA 加合物的研究，是癌症病因学的一个重要领域。对动物和人类体内形成的低丰度 DNA 加合物的分析需要开发和实施高度灵敏的分析方法。由于只能分离出微量 DNA（约 100 杯），因此显然每次分析消耗的样品量应尽可能少，以便收集尽可能多的分析信息。本文给出了一个示例，说明如何通过使用毛细管液相色谱与电喷雾串联质谱联用实施数据相关采集来解决此问题。作为案例研究，介绍了美法仑对 DNA 的烷基化。稍微修改色谱条件（流动相和固定相）即可自动分析其他种类的 DNA 加合物。版权所有 (C) 2004 John Wiley Sons, Ltd.
The study of defects in DNA caused by xenobiotics, more particularly the study of DNA adducts, is an important field in cancer aetiology. The analysis of low abundance DNA adducts formed in vivo both in animals and humans requires the development and implementation of highly sensitive analytical methods. Since only a minute amount of DNA can be isolated (ca. 100 mug) it is evident that the amount of sample consumed per analysis should be as small as possible in order to gather as much analytical information as possible. In this article an example is given of how this problem can be solved by the implementation of data-dependent acquisitions using capillary liquid chromatography coupled to electrospray tandem mass spectrometry. As a case study the alkylation of DNA by melphalan is presented. Slight modifications of the chromatographic conditions (mobile and stationary phases) can allow the automated analysis of other kinds of DNA adducts. Copyright (C) 2004 John Wiley Sons, Ltd.