Measurement and analysis of fluorescence decay curves
Measurement and analysis of fluorescence decay curves
复制标题
荧光衰减曲线的测量与分析
DOI：
10.1063/1.1134962
复制
发表时间：
1977
影响因子：
1.6
通讯作者：
H. P. Good
中科院分区：
文献类型：
--
作者：
U. Wild;A. Holzwarth;H. P. Good
介绍了一种以锁模Ar离子激光器为激发源，采用单光子计数探测的荧光寿命装置。激励源的高重复率和高强度允许在停止通道中进行双重辨别，从而提供出色的时间分辨率。数据分析以统计概念为基础。讨论了去卷积衰减曲线的傅里叶变换的标准差，并概述了在傅里叶空间进行曲线拟合的优点。
A fluorescence lifetime apparatus using a mode‐locked argon ion laser as excitation source and single photon counting detection is described. The high repetition rate and intensity of the excitation source allows twofold discrimination in the stop channel, giving excellent time resolution. The data analysis is based on statistical concepts. The standard deviations of the Fourier transforms of the deconvolved decay curves are discussed and the advantages of performing a curve fit in the Fourier space are outlined.