Comparative Assessment of Sera from Individuals after S-Gene RNA-Based SARS-CoV-2 Vaccination with Spike-Protein-Based and Nucleocapsid-Based Serological Assays.
Comparative Assessment of Sera from Individuals after S-Gene RNA-Based SARS-CoV-2 Vaccination with Spike-Protein-Based and Nucleocapsid-Based Serological Assays.
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DOI：
10.3390/diagnostics11030426
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发表时间：
2021-03-03
期刊：
影响因子：
--
通讯作者：
Zautner AE
中科院分区：
文献类型：
--
作者：
Dörschug A;Frickmann H;Schwanbeck J;Yilmaz E;Mese K;Hahn A;Groß U;Zautner AE
由于开始接种COVID-19疫苗，疫苗相关体液应答与基于血清学的SARS-CoV-2自然感染监测以及突破性感染之间的血清学区分成为一个相关问题。在这里，我们评估了使用SARS-CoV-2刺突蛋白表位的RNA疫苗应用对基于抗刺突蛋白的血清学（EUROIMMUN）和基于抗核衣壳的血清学（VIROTECH）结果的差异化影响。对接种后不同时间点采集的80份疫苗接种者血清样本进行了评估。虽然在所有样本中均观察到抗加标蛋白试验中的阳性或临界血清学应答（IgG和伊加均为90%，仅IgA为6.3%，仅IgG为3.8%），但由于抗核衣壳试验的特异性，如预期，仅观察到一例IgM假阳性。在第一剂疫苗接种后第二周就已经可检测到阳性抗刺突蛋白抗体，第二剂疫苗接种后滴度更高。总之，基于抗刺突蛋白的血清学和基于抗核衣壳的血清学的联合应用将为区分疫苗接种应答和自然感染提供有用的选择。
Due to the beginning of vaccination against COVID-19, serological discrimination between vaccine-associated humoral response and serology-based surveillance of natural SARS-CoV-2 infections as well as breakthrough infections becomes an issue of relevance. Here, we assessed the differentiated effects of the application of an RNA vaccine using SARS-CoV-2 spike protein epitopes on the results of both anti-spike protein–based serology (EUROIMMUN) and anti-nucleocapsid-based serology (VIROTECH). A total of 80 serum samples from vaccinees acquired at different time points after vaccination was assessed. While positive or borderline serological response in the anti-spike protein assay was observed for all samples (90% both IgG and IgA, 6.3% IgA only, 3.8% borderline IgG only), only a single case of a falsely positive IgM was observed for the anti-nucleocapsid assay as expected due to this assay’s specificity. Positive anti-spike protein antibodies were already detectable in the second week after the first dose of vaccination, with higher titers after the second dose of the vaccine. In conclusion, the combined application of anti-spike protein–based serology and anti-nucleocapsid-based serology will provide a useful option for the discrimination of vaccination response and natural infection.
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DOI：
10.1016/s1473-3099(20)30699-x
发表时间：
2020-12
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Houlihan CF;Beale R
通讯作者：
Beale R
影响因子：
4.1
作者：
Herrera NG;Morano NC;Celikgil A;Georgiev GI;Malonis RJ;Lee JH;Tong K;Vergnolle O;Massimi AB;Yen LY;Noble AJ;Kopylov M;Bonanno JB;Garrett-Thomson SC;Hayes DB;Bortz RH 3rd;Wirchnianski AS;Florez C;Laudermilch E;Haslwanter D;Fels JM;Dieterle ME;Jangra RK;Barnhill J;Mengotto A;Kimmel D;Daily JP;Pirofski LA;Chandran K;Brenowitz M;Garforth SJ;Eng ET;Lai JR;Almo SC
通讯作者：
Almo SC
影响因子：
8
作者：
McAndrews, Kathleen M.;Dowlatshahi, Dara P.;Kalluri, Raghu
通讯作者：
Kalluri, Raghu
影响因子：
4.8
作者：
Byrnes, James R.;Zhou, Xin X.;Wells, James A.
通讯作者：
Wells, James A.
影响因子：
12.7
作者：
Kohmer, Niko;Westhaus, Sandra;Rabenau, Holger F.
通讯作者：
Rabenau, Holger F.