Candida albicans Adheres to Chitin by Recognizing N-acetylglucosamine (GlcNAc).
Candida albicans Adheres to Chitin by Recognizing N-acetylglucosamine (GlcNAc).
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DOI：
10.3314/mmj.16-00007
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发表时间：
2017
影响因子：
1
通讯作者：
S. Ishijima;Tsuyoshi Yamada;Naho Maruyama;S. Abe
中科院分区：
文献类型：
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作者：
S. Ishijima;Tsuyoshi Yamada;Naho Maruyama;S. Abe
用细胞结合试验检测白念珠菌细胞与甲壳素的结合情况。显微镜观察表明，活的和热灭活的念珠菌细胞都结合在甲壳素涂层的底物上。白色念珠菌优先结合在甲壳素涂层的塑料平板上，而不是壳聚糖涂层的和未涂层的平板上。我们制备了125I标记的念珠菌细胞，用于定量分析它们与甲壳素的结合。热灭活的~(125)I标记的念珠菌细胞以时间依赖的方式结合在甲壳素涂层的平板上，直到4℃开始孵育1.5h。加入未标记的活的或未标记的热灭活的念珠菌细胞，可抑制~(125)I标记的念珠菌细胞与甲壳素涂层平板的结合。添加25 mg/ml甲壳素或10%以下的壳聚糖也可降低念珠菌与甲壳素的结合力。甲壳素的主要成分N-乙酰氨基葡萄糖(GlcNAc)在12.5~200 mM范围内呈剂量依赖性抑制念珠菌与甲壳素的结合。壳聚糖的一种成分氨基葡萄糖则没有这种抑制作用。这些发现表明，念珠菌与甲壳素的结合可能是通过识别GlcNAc来实现的。
The binding of Candida albicans cells to chitin was examined in a cell-binding assay. Microscopic observations indicated that both living and heat-killed Candida cells bound to chitin-coated substrates. C. albicans preferentially bound to chitin-coated plastic plates over chitosan-coated and uncoated plates. We prepared 125I-labeled Candida cells for quantitative analysis of their binding to chitin. Heat-killed 125I-labeled Candida cells bound to chitin-coated plates in a time-dependent manner until 1.5 hours after start of incubation at 4℃. The binding of 125I-labeled Candida cells to chitin-coated plates was inhibited by adding unlabeled living or unlabeled heat-killed Candida cells. The binding of Candida to chitin was also reduced by addition of 25 mg/ml chitin or chitosan up to 10%. N-acetylglucosamine (GlcNAc), which is a constituent of chitin, inhibited binding of Candida to chitin in a dose-dependent manner between 12.5 and 200 mM. Glucosamine, which is a constituent of chitosan, showed no such inhibitory effect. These findings suggest that the binding of Candida to chitin may be mediated by recognition of GlcNAc.