ISOLATION AND STRUCTURE ELUCIDATION OF A NOVEL ADIPOKINETIC HORMONE (LOM-AKH-III) FROM THE GLANDULAR LOBES OF THE CORPUS CARDIACUM OF THE MIGRATORY LOCUST, LOCUSTA-MIGRATORIA
ISOLATION AND STRUCTURE ELUCIDATION OF A NOVEL ADIPOKINETIC HORMONE (LOM-AKH-III) FROM THE GLANDULAR LOBES OF THE CORPUS CARDIACUM OF THE MIGRATORY LOCUST, LOCUSTA-MIGRATORIA
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DOI：
10.1111/j.1432-1033.1991.tb15713.x
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发表时间：
1991-01-30
期刊：
影响因子：
--
通讯作者：
BEENAKKERS, AMT
中科院分区：
文献类型：
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作者：
OUDEJANS, RCHM;KOOIMAN, FP;BEENAKKERS, AMT
从东亚飞蝗（Locusta migratoria）心体腺叶中分离到一种新的促脂肪运动激素（Lom-AKH-III）。 在N-末端，它被5-氧代脯氨酸（焦谷氨酸）残基（< Glu）封闭。 酶解封闭后，通过自动Edman降解部分建立N-末端的氨基酸序列为[Glu]-Leu-Asn-Phe-Thr-Pro-。 快原子轰击光谱法（FAB-MS）显示，新的激素是一个八肽，这是酰胺化的C-末端，并具有相对分子质量为1072。 基于FAB-MS数据，完整序列为< Glu-Leu-Asn-Phe-Thr-Pro-Trp-Trp-NH 2，其通过化学合成确认。 天然激素和合成肽的HPLC、FAB-MS和生物活性特征一致。 虽然这种新激素的结构类似于Lom-AKH-I（< Glu-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH 2），但其氨基酸序列指向其生物合成的完全不同的途径，涉及第三种激素原。 高[K+]含量的培养基可以在体外引起所有三种脂肪运动激素的释放。 有趣的是，另一种蝗虫Schistocerca gregaria中没有这种新的激素。根据体外生物合成实验，这种激素的周转率非常高，表明其具有重要的生理功能。 飞蝗是第一个被证实具有三种不同脂肪代谢激素的昆虫。
A new adipokinetic hormone (named Lom-AKH-III) was isolated from the glandular lobes of the corpora cardiaca of Locusta migratoria. At the N-terminus it is blocked by a 5-oxoproline (pyroglutamic acid) residue (< Glu). After enzymatic deblocking, the amino acid sequence of the N-terminus was partly established by automatic Edman degradation to be [< Glu]-Leu-Asn-Phe-Thr-Pro-. Fast-atom-bombardment spectrometry (FAB-MS) revealed that the new hormone is an octapeptide, which is amidated at the C-terminus, and has a relative molecular mass of 1072. Based on the FAB-MS data the complete sequence is < Glu-Leu-Asn-Phe-Thr-Pro-Trp-Trp-NH2, which was confirmed by chemical synthesis. All characteristics from HPLC, FAB-MS and biological activity of the natural hormone and the synthetic peptide appeared to be identical. Although the structure of this new hormone resembles that of Lom-AKH-I (< Glu-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2), its amino acid sequence points to a completely different route for its biosynthesis, involving a third prohormone. High-[K+]-containing media can cause release of all three adipokinetic hormones in vitro. Interestingly, the new hormone is absent in another locust species, Schistocerca gregaria. Based on in vitro biosynthesis experiments the turnover for this hormone is very high, suggesting an important physiological function. Locusta migratoria is the first insect species in which three different adipokinetic hormones have been demonstrated.