Multispectral bioluminescence tomography: methodology and simulation.
Multispectral bioluminescence tomography: methodology and simulation.
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DOI：
10.1155/ijbi/2006/57614
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发表时间：
2006
影响因子：
7.6
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Cong AX;Wang G
生物发光成像已被证明是一种有价值的工具，用于监测活的小动物在细胞和分子水平上的生理和病理活动。使用生物技术，可以用编码几种荧光素酶的报告基因标记靶细胞，所述荧光素酶在覆盖红外范围的宽光谱中产生特征光子。部分漫射光可以到达小动物的身体表面，使用适当的滤光片将其分成几个光谱带，并由灵敏的CCD相机收集。在这里，我们提出了一种生物发光断层扫描（BLT）的方法，从外表面上测量的多光谱数据的生物发光源重建，并证明了多光谱BLT的优点，在数值研究中使用的异质性小鼠胸部幻影。结果表明，多光谱的方法显着提高了BLT重建的精度和稳定性，即使数据是高噪声。
Bioluminescent imaging has proven to be a valuable tool for monitoring physiological and pathological activities at cellular and molecular levels in living small animals. Using biological techniques, target cells can be tagged with reporters encoding several kinds of luciferase enzymes, which generate characteristic photons in a wide spectrum covering the infrared range. Part of the diffused light can reach the body surface of the small animal, be separated into several spectral bands using appropriate filters, and collected by a sensitive CCD camera. Here we present a bioluminescence tomography (BLT) method for a bioluminescent source reconstruction from multispectral data measured on the external surface, and demonstrate the advantages of multispectral BLT in a numerical study using a heterogeneous mouse chest phantom. The results show that the multispectral approach significantly improves the accuracy and stability of the BLT reconstruction even if the data are highly noisy.