Development and evaluation of a rotary cell for capillary electrophoresis-chemiluminescence detection.
Development and evaluation of a rotary cell for capillary electrophoresis-chemiluminescence detection.
复制标题
DOI：
10.1021/ac100007d
复制
发表时间：
2010-05
影响因子：
7.4
通讯作者：
Junhua Wang;Linmei Li;Weihua Huang;Jieke Cheng
中科院分区：
文献类型：
--
作者：
Junhua Wang;Linmei Li;Weihua Huang;Jieke Cheng
通过不断开发和改进界面，毛细管电泳法化学发光(CE-CL)检测及其应用得到了很大的发展。在本研究中，制作了一种用于CE-CL检测的新型旋转池，并对其进行了评价。在胞体中制作了一条底部为石英的环形窄通道，用于容纳CL反应物，并作为反应室。毛细管电泳管放置在靠近反应室底部的位置，分析物在此沉积到CL反应物中以进行反应。检测是通过反应通道下方的光电倍增管实现的。旋转反应池的一个优点是，在实验过程中，当它以预设速度旋转时，它更新毛细管端的反应物。旋转检测池解决了经常中断电泳的气泡形成和流动堵塞的问题，为传统的同轴流构型提出了一个新的概念。以辣根过氧化物酶(HRP)和血红蛋白(Hb)为标准蛋白质，评价了鲁米诺/H(2)O(2)CL体系的界面性能。在这项概念验证实验中，获得了满意的灵敏度(S/N=3，HRP和Hb的LOD值分别为0.91×10(-9)M和4.37×10(-8)M)。这种新颖的设计为CE-CL联用提供了一个简单而健壮的接口。
Many efforts have been made toward the advancement of capillary electrophoresis chemiluminescence (CE-CL) detection and its applications through continuous development and improvement of interfaces. In this study, a novel rotary cell for CE-CL detection was fabricated and evaluated. A ring-shaped narrow channel with a quartz bottom is made in a cell body to hold CL reactants and act as the reaction chamber. The CE capillary is placed closely to the bottom of the reaction chamber where analyte is deposited into the CL reactants for reactions to occur. Detection is achieved with a photomultiplier tube below the reaction channel. An advantage of the rotary reaction cell is that it renews the reactants at the capillary end as it revolves at a preset speed during experiments. The rotary detection cell presents a new concept to the conventional coaxial-flow configuration by solving the problems of bubble formation and flow blockage that often interrupt the electrophoresis. Two standard proteins, horseradish peroxidase (HRP) and hemoglobin (Hb), were used to evaluate the interface's performance with luminol/H(2)O(2) CL system. Satisfactory sensitivities (LOD of 0.91 x 10(-9) M for HRP, and 4.37 x 10(-8) M for Hb at S/N = 3) were obtained in this proof-of-concept experiment. This novel design provides a straightforward and robust interface for CE-CL hyphenation.