Screen filtration pressure (SFP)法による血小板凝集の測定について
Screen filtration pressure (SFP)法による血小板凝集の測定について
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使用筛滤压力（SFP）法测量血小板聚集
DOI：
10.11406/rinketsu.14.43
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
藤江 沼野
中科院分区：
文献类型：
--
作者：
博男 山崎;藤江 沼野
ADP 引起的血小板聚集通过 Swank (1961) 描述的筛网过滤压力 (SFP) 方法进行测量。当血液以稳定的速率被迫通过具有多个 20×20 微米方形微孔、深度约为 20 微米的筛网时，SFP 装置测量以毫米汞柱为单位的流体流动阻力。 SFP方法使用全血，因此可以避免使用该方法在离心收集富含血小板的血浆时可能发生的血小板破坏或损伤。使用 19 名人类受试者，年龄从 24 岁到 60 岁，其中 11 名男性和 8 名女性。添加1/10体积的3×10-5摩尔ADP的血液的SFP变化均小于平均值的20％。因此SFP方法的重复变异性并不那么高，SFP方法可能有临床应用价值。在使用过的筛子的孔中检测到大量聚集的血小板团和少量红细胞。研究结果表明，SFP 主要是由于血液中聚集的血小板质量的等级所致。讨论了可能影响SFP 的其他因素。
Platalet aggregation by ADP was measured by screen filtration pressure (SFP) method, which was described by Swank (1961). The SFP apparatus measures the resistance to flow of a fluid in mmHg as the blood is forced at a steady rate through a screen with multiple micro-pores 20× 20 micra square and approximately 20 micra deep. Whole blood was used in SFP method, so destruction or injury of plateles which may occur during centrifugation to collect platelet rich plasma are able to avoid using this method. Nineteen human subjects, aging from 24 to 60 years, 11 males and 8 females, were used. Variations of SFP of blood added with 1/10 volume of 3× 10-5 molar of ADP were less than 20 percent of the average value in all cases. So the repeated variability of SFP method was not so high and SFP method may be valuable for clinical use. In pores of used screen large number of aggregated platelets masses and few red blood cells were detected. The finding suggests that SFP is mainly due to grade of aggregated platelet mass in blood. Other factors which may influence of SFP were discussed.