Plasma quercetin metabolites are affected by intestinal microbiota of human microbiota-associated mice fed with a quercetin-containing diet
Plasma quercetin metabolites are affected by intestinal microbiota of human microbiota-associated mice fed with a quercetin-containing diet
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DOI：
10.3164/jcbn.19-45
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发表时间：
2019-10
影响因子：
2.4
通讯作者：
M. Tamura;H. Nakagawa;S. Hori;Tadahiro Suzuki;K. Hirayama
中科院分区：
文献类型：
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作者：
M. Tamura;H. Nakagawa;S. Hori;Tadahiro Suzuki;K. Hirayama
据报道，槲皮素对高脂饮食引起的小鼠非酒精性脂肪肝具有保护作用。最近的研究表明，几种肠道细菌可以代谢槲皮素。我们假设肠道微生物群组成的差异会影响肠道对槲皮素的吸收。将无菌 BALB/cA 雌性小鼠（18 周龄）随机分为 4 组，并口服 4 个人类个体（HF1、HF2、HF3、HF4）的粪便悬浮液，以产生人类微生物群相关小鼠。所有小鼠均喂食含 0.05% 槲皮素的颗粒饲料 4 周。四组之间血浆总胆固醇和盲肠微生物群存在显着差异。 HF3 组的血浆槲皮素浓度显着高于 HF1 组。血浆异鼠李素/槲皮素比率与内脏脂肪水平呈显着负相关（r = -0.544，p = 0.013）。 Log10肠杆菌计数与血浆槲皮素代谢物呈正相关。主成分分析通过第二、第三主成分的相关图显示各组分布在不同的区域。这项研究表明，接种不同粪便悬浮液的人类微生物相关小鼠的肠道微生物对膳食槲皮素的反应不同。
Protective effect of quercetin on high-fat diet-induced non-alcoholic fatty liver disease in mice has been reported. Recent research has revealed that several intestinal bacteria metabolize quercetin. We hypothesize that the difference in composition of intestinal microbiota affects quercetin absorption from the intestine. Germ-free BALB/cA female mice (18 weeks old) were randomly divided into four groups and orally administered with fecal suspension from four human individuals (HF1, HF2, HF3, HF4) to produce the human microbiota-associated mice. All mice were fed the 0.05% quercetin-containing pelleted diet for four weeks. Significant differences were observed in plasma total cholesterol and cecal microbiota among the four groups. Plasma quercetin concentration was significantly higher in the HF3 group than in the HF1 group. The plasma isorhamnetin/quercetin ratio showed significant negative correlation with visceral fat levels (r = −0.544, p = 0.013). Positive correlation was observed between the Log10 Enterobacteriaceae count and the plasma quercetin metabolites. Principal component analysis showed that all groups were distributed in different regions by using the correlation diagram with the second and third principal component. This study indicates that intestinal microbiota of human microbiota-associated mice inoculated with different fecal suspensions react to dietary quercetin in different ways.