Chimeric AGAMOUS repressor induces serrated petal phenotype in Torenia fournieri similar to that induced by cytokinin application
Chimeric AGAMOUS repressor induces serrated petal phenotype in Torenia fournieri similar to that induced by cytokinin application
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DOI：
10.5511/plantbiotechnology.25.45
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Ohtsubo, Norihiro
中科院分区：
文献类型：
--
作者：
Narumi, Takako;Aida, Ryutaro;Ohtsubo, Norihiro
同源异型蛋白AGAMOUS（AG）终止拟南芥花分生组织，促进雄蕊和心皮的发育。嵌合AG阻遏物（AGSRDX）的功能或表达的破坏导致多余的花瓣，称为重瓣表型。为了研究拟南芥中的这种形态变化是否适用于观赏花卉以增加其园艺价值，我们将AGSRDX引入蓝猪耳（Torenia fournieri Lind.）植物表达AGSRDX的转基因蓝猪耳植株在花瓣数上没有冗余，但花瓣边缘出现锯齿，柱头表面出现花色素苷积累和形态变化，花瓣和花柱中形成额外的维管束。解剖学观察表明，这些表型与氯吡脲（CPPU）处理的蓝猪耳属植物的表型高度相似，特别是在维管束的紊乱。当蓝猪耳C功能基因TfFAR或TfPLE1（金鱼草FARINELLI和PLENA的同源物）的嵌合阻遏物表达时，也观察到类似于AGSRDX转基因蓝猪耳植物的表型。这些结果表明，AGSRDX转基因蓝猪耳植株的形态变化是由C功能的破坏引起的。这些新的表型可能是由花椰菜花叶病毒（CaMV）35S启动子的细胞分裂素依赖的调节维管束形成和异位表达的嵌合阻遏物在所有轮的修改。
The homeotic protein AGAMOUS (AG) terminates the floral meristem and promotes the development of stamens and carpels in Arabidopsis. Disruption of its function or expression of the chimeric AG repressor (AGSRDX) results in redundant petals, known as a double flower phenotype. To investigate whether this morphological change in Arabidopsis is applicable to ornamental flowers to increase their horticultural value, we introduced AGSRDX into torenia (Torenia fournieri Lind.) plants. Transgenic torenia plants expressing AGSRDX showed no redundancy in petal number, although they exhibited serration in petal margins, anthocyanin accumulation and morphological change in the stigma surface, and formation of extra vascular bundles in petals and styles. Anatomical observation of petals and styles revealed that these phenotypes are highly similar to those of forchlorfenuron (CPPU)-treated torenia plants especially in the derangement of vascular bundles. Phenotypes similar to AGSRDX transgenic torenia plants were also observed when the chimeric repressors for torenia C-function genes TfFAR or TfPLE1, homologs of Antirrhinum FARINELLI and PLENA respectively, were expressed. These results suggest that the morphological changes in AGSRDX transgenic torenia plants are induced by the disruption of C-function. These novel phenotypes might be caused by the modification of cytokinin-dependent regulation in vascular bundle formation and ectopic expression of the chimeric repressors in all whorls by the cauliflower mosaic virus (CaMV) 35S promoter.