CUCUMBER MOSAIC-VIRUS SATELLITE RNA (Y-STRAIN) - ANALYSIS OF SEQUENCES WHICH AFFECT YELLOW MOSAIC SYMPTOMS ON TOBACCO
CUCUMBER MOSAIC-VIRUS SATELLITE RNA (Y-STRAIN) - ANALYSIS OF SEQUENCES WHICH AFFECT YELLOW MOSAIC SYMPTOMS ON TOBACCO
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DOI：
10.1099/0022-1317-71-9-1905
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发表时间：
1990-09-01
影响因子：
3.8
通讯作者：
BAULCOMBE, D
中科院分区：
文献类型：
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作者：
JAEGLE, M;DEVIC, M;BAULCOMBE, D
黄瓜花叶病毒（CMV）（KIN株）感染烟草产生轻度花叶病，而CMV卫星RNA（Y株）感染烟草产生严重的黄色花叶。分析重组和突变形式的卫星RNA确定了一个网站（核苷酸185/186）的Y卫星RNA，影响的能力，诱导黄色花叶病毒与CMV的组合，但不与番茄无精病毒。该位点相对于卫星RNA中其他突变的位置表明，可能由卫星RNA编码的多肽在诱导黄色花叶症状中没有作用。因此，症状诱导是卫星RNA在辅助病毒的干预下对宿主植物的影响。在卫星RNA的突变分析过程中，我们检测到了几个在植物中出现的继发突变。其中两个是超过80个核苷酸的缺失。其他形式的突变卫星RNA是无功能的，即使修饰涉及核苷酸完全在大的二级缺失。这些数据意味着卫星RNA中复杂的分子内相互作用。
Plants infected with cucumber mosiac virus (CMV) (KIN strain) produced a mild mosaic disease on tobacco whereas infection of CMV with satellite RNA (strain Y) cause a severe yellow mosaic. Analysis of recombinant and mutant forms of satellite RNA identified a site (nucleotides 185/186) in the Y satellite RNA that affects the ability to induce the yellow mosaic in combination with CMV but not with tomato aspermy virus. The location of this site with respect to other mutations in the satellite RNA indicated that polypeptides, which may be encoded by the satellite RNA, have no role in induction of yellow mosaic symptoms. The symptom induction is therefore an effect of the satellite RNA on the host plant with the intervention of the helper virus. In the course of the mutation analysis of satellite RNA we detected several secondary mutations which arose in planta. Two of these were deletions of more than 80 nucleotides. Other forms of mutant satellite RNA were non-functional even though the modifications involved nucleotides completely within the large secondary deletions. These data imply complex intramolecular interactions in the satellite RNA.