The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion
The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion
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DOI：
10.1128/iai.66.3.1000-1007.1998
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发表时间：
1998-03-01
影响因子：
3.1
通讯作者：
Ramphal, R
中科院分区：
文献类型：
--
作者：
Arora, SK;Ritchings, BW;Ramphal, R
铜绿假单胞菌的粘蛋白特异性黏附在该菌最初在囊性纤维化患者的呼吸道定植中起重要作用。本文报道鞭毛帽蛋白Flid参与了这种黏附过程，在体外粘液黏附实验中，铜绿假单胞菌fld基因的一个极染色体插入突变使该细菌与粘蛋白不能黏附。通过在多拷贝质粒上单独提供fld基因，恢复了粘附性表型，这表明该基因参与了铜绿假单胞菌的粘蛋白粘附性。进一步的体外竞争实验表明，纯化的Flid蛋白抑制了非纤毛铜绿假单胞菌PAK-NP的粘蛋白黏附，而相同浓度的PilA和FLAG蛋白对这一功能没有影响。研究了Flid基因的调控，发现Flid基因Ras的转录不依赖于鞭毛Sigma因子(28)。与这一发现一致的是，在Flid启动子区域没有发现sigma(28)结合序列。β-半乳糖苷酶检测结果表明，铜绿假单胞菌中的Flid基因受新发现的转录调控因子FleQ和交替的sigma因子sigma(54)(RpoN)调控。
Mucin-specific adhesion of Peudomonas aeruginosa plays an important role in the initial colonization of this organism in the airways of cystic fibrosis patients, We report here that the flagellar cap protein, FliD, participates in this adhesion process, A polar chromosomal insertional mutation in the P. aeruginosa fliD gene made this organism nonadhesive to mucin in an in vitro mucin adhesion assay. The adhesive phenotype was restored by providing the fliD gene alone on a multicopy plasmid, suggesting involvement of this gene in mucin adhesion af P. aeruginosa. Further supporting this observation, the in vitro competition experiments demonstrated that purified FliD protein inhibited the mucin adhesion of nonpiliated P. aeruginosa PAK-NP, while the same concentrations of PilA and FlaG proteins of P. aeruginosa were ineffective in this function. The regulation of the fliD gene was studied anti was found to he unique in that the transcription of the fliD gene Ras independent of the flagellar sigma factor sigma(28). Consistent with this finding, no sigma(28) binding sequence could be identified in the fliD promoter region. The results of the beta-galactosidase assays suggest that the fliD gene in P. aeruginosa is regulated by the newly described transcriptional regulator FleQ and the alternate sigma factor sigma(54) (RpoN).