Shifts in circulating lymphocyte subsets in cats with feline infectious peritonitis (FIP): pathogenic role and diagnostic relevance
Shifts in circulating lymphocyte subsets in cats with feline infectious peritonitis (FIP): pathogenic role and diagnostic relevance
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DOI：
10.1016/s0165-2427(03)00156-9
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发表时间：
2003-12-15
影响因子：
1.8
通讯作者：
Poli, G
中科院分区：
文献类型：
--
作者：
Paltrinieri, S;Ponti, W;Poli, G
患有猫传染性腹膜炎（FIP）的猫通常淋巴细胞减少，脾脏和淋巴结中淋巴细胞明显减少。特别是，在FIP病变的发展过程中，组织中CD 4（+）淋巴细胞的数量减少。这种降低很可能是由于淋巴细胞凋亡率增加。相比之下，感染猫冠状病毒（FCoV）的猫在外周淋巴结中出现滤泡增生。本研究旨在评估FIP中循环淋巴细胞亚群变化的可能致病作用。对患有FIP的猫的外周血进行评价，并与生活在无FCoV和FCoV流行猫舍中的临床健康猫的外周血进行比较。还检查了患有FIP以外疾病的猫的血液，以确定变化的诊断相关性。采用全血间接免疫荧光技术和猫CD 5、CD 4、CD 8、CD 21特异性mAb，通过流式细胞术分析淋巴细胞亚群。目前的研究结果表明，最近感染FCoV的猫没有发展这种疾病，T细胞有短暂的增加;来自FIP高患病率组的猫T细胞亚群有中度但持续的减少; FIP猫的所有淋巴细胞亚群都有非常严重的减少。此外，在FIP过程中，许多淋巴细胞不表达任何膜抗原，最有可能是由于早期凋亡。患有FIP以外疾病的猫也有淋巴细胞数量减少：因此，这些发现的诊断相关性非常低。尽管如此，缺乏流式细胞术变化具有很高的阴性预测值（NPV），因此可以将FIP从细胞图正常的猫的可能诊断列表中排除。(C)2003 Elsevier B. V.保留所有权利。
Cats with feline infectious peritonitis (FIP) are usually lymphopenic and have lymphoid depletion evident in spleen and lymph nodes. In particular, the number of CD4(+) lymphocytes in tissues decreases during the evolution of FIP lesions. This decrease is most likely due to increased lymphocyte apoptotic rate. In contrast, cats infected with the Feline Coronavirus (FCoV) develop a follicular hyperplasia in the peripheral lymph nodes. The current study was devised to evaluate the possible pathogenic role of shifts in circulating lymphocyte subsets in FIP. Peripheral blood from cats with FIP was evaluated and compared with peripheral blood from clinically healthy cats living in both FCoV-free and FCoV-endemic catteries. Blood from cats with diseases other than FIP was also examined in order to define the diagnostic relevance of the changes. Lymphocyte subsets were analysed by flow cytometry, using a whole blood indirect immuno fluorescence technique and mAbs specific for feline CD5, CD4, CD8, CD21. The results of the current study suggest that cats recently infected with FCoV that do not develop the disease have a transient increase in T cells; cats from groups with high prevalence of FIP have a moderate but persistent decrease in T cell subsets; cats with FIP have a very severe decrease in all the subsets of lymphocytes. Moreover, during FIP many lymphocytes do not express any membrane antigen, most likely due to early apoptosis. Cats with diseases other than FIP also had decreased number of lymphocytes: as a consequence, the diagnostic relevance of these findings is very low. Nevertheless, the lack of flow cytometric changes had a high negative predictive value (NPV), thus allowing to exclude FIP from the list of possible diagnoses in cats with normal cytograms. (C) 2003 Elsevier B.V. All rights reserved.