How does Trypanosoma equiperdum fit into the Trypanozoon group?: A cluster analysis by RAPD and multiplex-endonuclease genotyping approach
How does Trypanosoma equiperdum fit into the Trypanozoon group?: A cluster analysis by RAPD and multiplex-endonuclease genotyping approach
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DOI：
10.1017/s0031182003002968
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发表时间：
2003-05-01
期刊：
影响因子：
2.4
通讯作者：
Büscher, P
中科院分区：
文献类型：
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作者：
Claes, F;Agbo, EC;Büscher, P
致病性伊氏锥虫、伊氏锥虫和布氏锥虫在形态上是相同的。在马身上，这些寄生虫被认为分别导致杜林、苏拉和纳格纳。以前区分这些种的分子尝试对于伊氏锥虫和马尾松都不成功；只有布氏锥虫可以在一定程度上分化。本研究利用随机扩增多态DNA技术和改良的AFLP技术，对10个马尾藻、8个伊氏锥虫和4个布氏锥虫进行了遗传多样性分析。所获得的结果证实了所测试的伊氏锥虫组的同质性。布氏分枝杆菌聚集在一个异质群中。对于马兜铃虫，情况更为复杂：10个马兜铃虫中有8个与伊氏锥虫群聚在一起，而2个马兜铃虫菌株与布氏锥虫关系更近。因此，可以提出两个假设：(1)只有2株马兜铃虫是真正的引起杜林的马兜铃虫；其他所有的马兜铃虫菌株实际上都是引起苏拉的伊氏锥虫；(2)马兜铃虫根本不存在。在这种情况下，马匹感染伊氏锥虫或布鲁氏锥虫的不同临床结果主要与宿主的免疫反应有关。
The pathogenic trypanosomes Trypanosoma equiperdum, T. evansi as well as T. brucei are morphologically identical. In horses, these parasites are considered to cause respectively dourine, surra and nagana. Previous molecular attempts to differentiate these species were not successful for T. evansi and T. equiperdum; only T. b. brucei could be differentiated to a certain extent. In this study we analysed 10 T. equiperdum, 8 T. evansi and 4 T. b. brucei using Random Amplified Polymorphic DNA (RAPD) and multiplex-endonuclease fingerprinting, a modified AFLP technique. The results obtained confirm the homogeneity of the T. evansi group tested. The T. b. brucei clustered out in a heterogenous group. For T. equiperdum the situation is more complex: 8 out of 10 T. equiperdum clustered together with the T. evansi group, while 2 T. equiperdum strains were more related to T. b. brucei. Hence, 2 hypotheses can be formulated: (1) only 2 T. equiperdum strains are genuine T. equiperdum causing dourine; all other T. equiperdum strains actually are T. evansi causing surra or (2) T. equiperdum does not exist at all. In that case, the different clinical outcome of horse infections with T. evansi or T. b. brucei is primarily related to the host immune response.