Comparative Evaluation of Chrysanthemum Flos from Different Origins by HPLC-DAD-MS n and Relative Response Factors
Comparative Evaluation of Chrysanthemum Flos from Different Origins by HPLC-DAD-MS n and Relative Response Factors
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HPLC-DAD-MSn比较评价不同产地菊花及相关响应因子
DOI：
10.1007/s12161-014-9864-0
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发表时间：
2015-01-01
影响因子：
2.9
通讯作者：
Song, Xiao-mei
中科院分区：
文献类型：
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作者：
Wang, Shan;Hao, Li-Juan;Song, Xiao-mei
菊花（Chrysanthemum Flos，CF）是菊科植物的干燥头状花序，在中国以及亚洲和东北欧的其他地区用于草药和茶材料。有证据表明，它可能具有抗氧化、抗炎和心脏保护功能。建立了高效液相色谱-二极管阵列检测器-电喷雾串联质谱（HPLC-DAD-ESI/MS）联用技术，利用相对响应因子（RRFs）对不同品种、不同品种、不同掺伪品等9个来源的CF进行了定性和定量分析。选择容易获得的活性成分绿原酸作为参考化合物，用于计算其他7种分析物的相对保留因子，包括毛地黄黄酮、槲皮素、毛地黄黄酮-7-O-葡萄糖苷、3，5-二咖啡酰喹啉酸、蒙花苷、芹菜素、薯蓣皂苷和金合欢素。在340 nm波长下，它们对绿原酸的相对保留因子在0.506和1.103之间。在三种色谱柱和两种高效液相色谱系统上的相对标准偏差小于3.8%，重现性好。使用RRF，CF的主要成分被量化。在此基础上，运用系统聚类分析（HCA）对不同来源的CF进行了分类。该方法适用于CF不同来源的快速鉴别。当黄酮类化合物和羟基肉桂酰奎尼酸的参比标准品不容易获得时，它对于CF的定量分析特别有用。
Chrysanthemum Flos (CF), the dry capitulum of the Compositae family of plants, is used in herbal medicine and as tea material in China, as well as other areas of Asia and northeastern Europe. Evidence indicates it may have antioxidant, anti-inflammatory, and cardioprotective function. We developed a HPLC coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MS) method to identify and characterize the main chemical components of CF from nine origins, including different species, cultivars, and adulterations, using relative response factors (RRFs). The readily available active component chlorogenic acid was selected as the reference compound for calculating the RRFs of the other seven analytes, including luteolin, quercetin, luteolin-7-O-glucoside, 3,5-dicaffeoylquninic acid, linarin, apigenin, diosmetin, and acacetin. Their RRFs against chlorogenic acid at 340 nm were between 0.506 and 1.103. The variation of RRFs on three columns and two HPLC systems was less than 3.8 %, with good reproducibility. Using the RRFs, the main components of CF were quantified. Furthermore, based on their quantitative comparison results, hierachical clustering analysis (HCA) was employed to differentiate the various origins of CF. The proposed method is appropriate for rapid discrimination of the different origins of CF. It is especially useful for the quantitative analysis of CF when the reference standards of flavonoids and hydroxycinnamoylquinic acids are not readily available.