QUANTIFICATION OF THE RELATIVE ABUNDANCE OF THE TOXIC DINOFLAGELLATE, KARENIA BREVIS (DINOPHYTA), USING UNIQUE PHOTOPIGMENTS 1
QUANTIFICATION OF THE RELATIVE ABUNDANCE OF THE TOXIC DINOFLAGELLATE, KARENIA BREVIS (DINOPHYTA), USING UNIQUE PHOTOPIGMENTS 1
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DOI：
10.1046/j.1529-8817.2003.01219.x
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发表时间：
2003-04
影响因子：
2.9
通讯作者：
E. Örnólfsdóttir;J. Pinckney;P. Tester
中科院分区：
文献类型：
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作者：
E. Örnólfsdóttir;J. Pinckney;P. Tester
诊断性光色素分析是确定天然浮游植物组合中藻类群的存在和相对丰度的有用工具。当一个属具有独特的光色素组成时，这种方法特别有用。有毒鞭毛藻短凯伦氏菌（Davis） G. Hansen & Moestrup梳子。11月与少数其他鞭毛藻共享诊断色素旋黄素-二酯，并且缺乏环甲素，这是大多数鞭毛藻物种的主要诊断色素之一。本研究利用化学分类程序（CHEMTAX）的初始色素比例矩阵，分析了短叶藻生物量在佛罗里达州和德克萨斯州加尔维斯顿湾混合河口浮游植物组合中的相对贡献。Galveston湾的浮游植物群落组成是基于细胞计数和生物体积测量以及生物量的chl - a特异性光色素估算（HPLC和CHEMTAX）计算的。对浮游植物群落结构的CHEMTAX和生物体积估计值没有显著差异，这表明HPLC-CHEMTAX方法可以合理地估计自然组合中的短叶矮蓟生物量。从加尔维斯顿湾采得的短叶蝉每细胞的旋黄质二酯含量明显高于从佛罗里达西海岸采得的短叶蝉。当应用适当的初始比例矩阵时，这种基于色素的方法为解决短叶藻华存在时浮游植物的时空分布提供了有用的工具。
Diagnostic photopigment analysis is a useful tool for determining the presence and relative abundance of algal groups in natural phytoplankton assemblages. This approach is especially useful when a genus has a unique photopigment composition. The toxic dinoflagellate Karenia brevis (Davis) G. Hansen & Moestrup comb. nov. shares the diagnostic pigment gyroxanthin‐diester with only a few other dinoflagellates and lacks peridinin, one of the major diagnostic pigments of most dinoflagellate species. In this study, measurements of gyroxanthin‐diester and other diagnostic pigments of K. brevis were incorporated into the initial pigment ratio matrix of the chemical taxonomy program (CHEMTAX) to resolve the relative contribution of K. brevis biomass in mixed estuarine phytoplankton assemblages from Florida and Galveston Bay, Texas. The phytoplankton community composition of the bloom in Galveston Bay was calculated based on cell enumerations and biovolumetric measurements in addition to chl a‐specific photopigment estimates of biomass (HPLC and CHEMTAX). The CHEMTAX and biovolume estimates of the phytoplankton community structure were not significantly different and suggest that the HPLC–CHEMTAX approach provides reasonable estimates of K. brevis biomass in natural assemblages. The gyroxanthin‐diester content per cell of K. brevis from Galveston Bay was significantly higher than in K. brevis collected from the west coast of Florida. This pigment‐based approach provides a useful tool for resolving spatiotemporal distributions of phytoplankton in the presence of K. brevis blooms, when an appropriate initial ratio matrix is applied.