EXCITOTOXIC ACID LESIONS OF THE PRIMATE SUBTHALAMIC NUCLEUS RESULT IN TRANSIENT DYSKINESIAS OF THE CONTRALATERAL LIMBS
EXCITOTOXIC ACID LESIONS OF THE PRIMATE SUBTHALAMIC NUCLEUS RESULT IN TRANSIENT DYSKINESIAS OF THE CONTRALATERAL LIMBS
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DOI：
10.1152/jn.1992.68.5.1850
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发表时间：
1992-11-01
影响因子：
2.5
通讯作者：
DELONG, MR
中科院分区：
文献类型：
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作者：
HAMADA, I;DELONG, MR
1.为了研究丘脑底核（subthalamic nucleus，简称丘脑底核）在运动障碍发病机制中的作用，本实验用保留纤维的兴奋性毒素损毁两只清醒猴的丘脑底核.采用记录-注射联合装置，在生理引导下准确定位、毁损椎间盘。将少量（1穆尔）的鹅膏蕈氨酸（10 μ g/穆尔）或红藻氨酸（1 μ g/穆尔）注射到脊髓中的4至7个靶位点中的每一个中。尸检组织学证实病变局限于延髓，每个部位每个病变的体积占核总体积的4-11%.每次注射结束后约20 min，注射部位对侧上肢和下肢远端部分的运动频率增加。60-80分钟后，对侧肢体出现严重运动障碍，近端关节受累程度大于远端关节，持续时间小于或等于4小时。每个病灶的受累模式和时间进程相似。在损伤后的几天内，在对侧的手和脚中仅观察到罕见的运动障碍，通常发生在动物受到刺激时。尽管有严重的运动障碍，但对自主运动（如理毛或伸手）没有明显影响。这些结果表明，减少的活动在运动障碍的生产中发挥了作用。
1. To examine the role of the subthalamic nucleus (STN) in the pathogenesis of dyskinesias, the STN was experimentally lesioned with fiber-sparing excitotoxins in two awake monkeys.2. A combined recording-injection device was used to locate and lesion the STN accurately under physiological guidance. A small amount (1 mul) of ibotenic acid (10 mug/mul) or kainic acid ( 1 mug/mul) was injected into each of four to seven target sites in the STN. Postmortem histology confirmed that lesions were confined to the STN, and the volume of each lesion at each site was 4-11% of the total volume of the nucleus.3. Approximately 20 min after the end of each injection, the frequency of movements increased in the distal portions of the upper and lower limbs contralateral to the injection site. Severe dyskinesias, involving the proximal joints to a greater degree than the distal, developed in the contralateral limbs after 60-80 min and lasted less-than-or-equal-to 4 h. The pattern of involvement and time course were similar after each lesion. In the days after the lesioning, only rare dyskinesias were observed in the contralateral hands and feet, typically occurring when the animals were stimulated.4. Despite the severe dyskinesias, there was no obvious effect on voluntary movements such as grooming or reaching.5. These results suggest that reduction of STN activity plays a role in the production of dyskinesias.