Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base-pair number after gel electrophoresis.
Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base-pair number after gel electrophoresis.
复制标题
凝胶电泳后蛋白质分子量和线性双链体 DNA 碱基对数标准曲线的稳健估计。
DOI：
10.1016/0003-2697(86)90420-3
复制
发表时间：
1986
影响因子：
2.9
通讯作者：
L. Mayer
中科院分区：
文献类型：
--
作者：
B. D. Plikaytis;G. Carlone;Paul Edmonds;L. Mayer
An accurate procedure for estimating linear-duplex DNA base-pair numbers and protein molecular weights after electrophoresis in single concentration gels is presented. A robust modified hyperbola was found to be superior for determining molecular weights and base-pair numbers for a set of known standards when compared with the conventional log transformation and a similar hyperbolic model. We describe the use of a soft laser-scanning densitometer to measure band-migration distances of wet, stained polyacrylamide gels for proteins and photograhic negatives of agarose gels containing DNA stained with ethidium bromide. This automated densitometric method was more accurate than existing methods. A BASIC computer program detailing the procedure is included.
